Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea

doi:10.1128/mSystems.00703-21

IOCAS-IR

	Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea
	Li, Lingyan 1,2; Zhang, Wenting 1,2; Zhang, Shengjie 1,2; Song, Lei 3; Sun, Qinglei4 ; Zhang, Huan 4; Xiang, Hua 1,2; Dong, Xiuzhu 1,2
	2021-09-01
发表期刊	MSYSTEMS
ISSN	2379-5077
卷号	6 期号:5 页码:16
通讯作者	Li, Lingyan([email protected]) ; Dong, Xiuzhu([email protected])
摘要	Cold seeps are globally widespread seafloor ecosystems that feature abundant methane production and flourishing chemotrophic benthic communities. Chemical evidence indicates that cold seep methane is largely biogenic; however, the primary methane-producing organisms and associated pathways involved in methano-genesis remain elusive. This work detected methane production when glycine betaine (GBT) or trimethylamine (TMA) was added to the sediment microcosms of the Formosa cold seep, South China Sea. The methane production was suppressed by antibiotic inhi-bition of bacteria, while GBT was accumulated. This suggests that the widely used osmoprotectant GBT could be converted to cold seep biogenic methane via the syner-gistic activity of bacteria and methanogenic archaea because archaea are not sensitive to antibiotics and no bacteria are known to produce ample methane (mM). 16S rRNA gene diversity analyses revealed that the predominant bacterial and archaeal gen-era in the GBT-amended methanogenic microcosms included Oceanirhabdus and Methanococcoides. Moreover, metagenomic analyses detected the presence of grdH and mtgB genes that are involved in GBT reduction and demethylation, respectively. Two novel species were obtained, including bacterium Oceanirhabdus seepicola, which reduces GBT to TMA, and a methanogenic archaeon, Methanococcoides seepicolus, which produces methane from TMA and GBT. The two strains reconstituted coculture efficiently converted GBT to methane at 18 degrees C; however, at 4 degrees C addition of dimethylgly-cine (DMG), the GBT demethylation product, was necessary. Therefore, this work dem-onstrated that GBT is the precursor not only of the biogenic methane but also of the cryoprotectant DMG to the microorganisms at the Formosa cold seep. IMPORTANCE Numerous cold seeps have been found in global continental margins where methane is enriched in pore waters that are forced upward from sediments. Therefore, high concerns have been focused on the methane-producing organisms and the metabolic pathways in these environments because methane is a potent greenhouse gas. In this study, GBT was identified as the main precursor for methane in the Formosa cold seep of the South China Sea. Further, synergism of bacteria and methanogenic archaea was identified in GBT conversion to methane via the GBT reduction pathway, while methanogen-mediated GBT demethylation to methane was also observed. In addition, GBT-demethylated product dimethyl glycine acted as a cryoprotectant that promoted the cold seep microorganisms at cold temperatures. GBT is an osmoprotectant that is widely used by marine organisms, and therefore, the GBT-derived methanogenic pathway reported here could be widely distributed among global cold seep environments.
关键词	cold seep GBT reduction and demethylation bacteria biogenic methane cryoprotectant dimethylglycine glycine betaine methanogenic archaea methanogenic precursor synergism
DOI	10.1128/mSystems.00703-21
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFC0310801]; Center for Ocean MegaScience, Chinese Academy of Sciences; Senior User Project of RV KEXUE[KEXUE2019GZ05]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000711932600007
出版者	AMER SOC MICROBIOLOGY
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177135
专题	中国科学院海洋研究所
通讯作者	Li, Lingyan; Dong, Xiuzhu
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, China Gen Microorganism Culture Collect Ctr, Inst Microbiol, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Lingyan,Zhang, Wenting,Zhang, Shengjie,et al. Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea[J]. MSYSTEMS,2021,6(5):16.
APA	Li, Lingyan.,Zhang, Wenting.,Zhang, Shengjie.,Song, Lei.,Sun, Qinglei.,...&Dong, Xiuzhu.(2021).Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea.MSYSTEMS,6(5),16.
MLA	Li, Lingyan,et al."Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea".MSYSTEMS 6.5(2021):16.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
mSystems.00703-21.pd（2590KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览