Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications

doi:10.3389/fmicb.2021.676447

	Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications
	Song, Huiyin 1,2,3; Chen, Yang1,2,3,4 ; Liu, Feng 1,2,3; Chen, Nansheng 1,2,3,5
	2021-07-02
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	12 页码:18
通讯作者	Chen, Nansheng([email protected])
摘要	The haptophyte Phaeocystis globosa is a well-known species for its pivotal role in global carbon and sulfur cycles and for its capability of forming harmful algal blooms (HABs) with serious ecological consequences. Its mitochondrial genome (mtDNA) sequence has been reported in 2014 but it remains incomplete due to its long repeat sequences. In this study, we constructed the first full-length mtDNA of P. globosa, which was a circular genome with a size of 43,585 bp by applying the PacBio single molecular sequencing method. The mtDNA of this P. globosa strain (CNS00066), which was isolated from the Beibu Gulf, China, encoded 19 protein-coding genes (PCGs), 25 tRNA genes, and two rRNA genes. It contained two large repeat regions of 6.7 kb and similar to 14.0 kb in length, respectively. The combined length of these two repeat regions, which were missing from the previous mtDNA assembly, accounted for almost half of the entire mtDNA and represented the longest repeat region among all sequenced haptophyte mtDNAs. In this study, we tested the hypothesis that repeat unit amplification is a driving force for different mtDNA sizes. Comparative analysis of mtDNAs of five additional P. globosa strains (four strains obtained in this study, and one strain previously published) revealed that all six mtDNAs shared identical numbers of genes but with dramatically different repeat regions. A homologous repeat unit was identified but with hugely different numbers of copies in all P. globosa strains. Thus, repeat amplification may represent an important driving force of mtDNA evolution in P. globosa.
关键词	mtDNA sizes mitochondrial genomes Phaeocystis globosa repeat region Haptophyta
DOI	10.3389/fmicb.2021.676447
收录类别	SCI
语种	英语
资助项目	National Key Research Development Program of China[2017YFC1404302]; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000]; Chinese Academy of Sciences Pioneer Hundred Talents Program; Taishan Scholar Project Special Fund; Qingdao Innovation and Creation Plan (Talent Development Program -5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16zhc]; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000674041600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177006
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Sch Earth & Planetary, Beijing, Peoples R China 5.Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC, Canada
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Song, Huiyin,Chen, Yang,Liu, Feng,et al. Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications[J]. FRONTIERS IN MICROBIOLOGY,2021,12:18.
APA	Song, Huiyin,Chen, Yang,Liu, Feng,&Chen, Nansheng.(2021).Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications.FRONTIERS IN MICROBIOLOGY,12,18.
MLA	Song, Huiyin,et al."Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications".FRONTIERS IN MICROBIOLOGY 12(2021):18.

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