Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus

doi:10.1016/j.aquaculture.2021.736960

	Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus
	Ding, Kui1,2,3,4,6 ; Zhang, Libin 1,2,3,4,5,6; Huo, Da 1,2,3,4,6; Guo, Xueying 1,2,3,4,6; Liu, Xiang 1,2,3,4,6; Zhang, Shuangli 1,2,3,4,6
	2021-10-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	543 页码:9
通讯作者	Zhang, Libin([email protected])
摘要	Evisceration is a peculiar behavior that happens frequently in the processes of aquaculture and transportation of sea cucumbers. This behavior involves a complex physiological process and results in the expulsion of the digestive tract and other viscera. However, studies on evisceration are insufficient, and the identification of the internal regulatory metabolites and potential pathways of evisceration in the sea cucumber are therefore likely to provide a scientific basis for this specific behavior. In this study, ultraperformance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was performed on the coelomic fluids that ejected by evisceration Apostichopus japonicus and in the coeloms of normal A. japonicus to detect changes in metabolites and metabolic pathways in the process of evisceration behavior. In total, nine metabolites were found to have increased and eleven to have decreased in the ejected coelomic fluids of evisceration sea cucumbers. These metabolites included phosphatidylethanolamine, glucosylceramide, L-tryptophan, carbamic acid, and cyclohexylamine. In addition, enrichment of metabolic pathway analyses revealed five significantly changed signaling pathways: Glycosyl-phosphatidyl-inositol (GPI)-anchor biosynthesis, regulation of autophagy, sphingolipid metabolism, nitrogen metabolism, and phenylalanine, tyrosine and tryptophan biosynthesis. These results contribute valuable data on the potential physiological mechanisms underlying evisceration behavior of A. japonicus, which could have important implications for the aquaculture and transportation of sea cucumbers.
关键词	Echinoderm Sea cucumber Evisceration Metabolite Metabolic pathway
DOI	10.1016/j.aquaculture.2021.736960
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876157]; Key Deployment Project of CAS Center for Ocean Mega-Science[COMS2019Q15]; Qingdao Postdoctoral Applied Research Project[2020170]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000672589300013
出版者	ELSEVIER
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176988
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Libin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ding, Kui,Zhang, Libin,Huo, Da,et al. Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus[J]. AQUACULTURE,2021,543:9.
APA	Ding, Kui,Zhang, Libin,Huo, Da,Guo, Xueying,Liu, Xiang,&Zhang, Shuangli.(2021).Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus.AQUACULTURE,543,9.
MLA	Ding, Kui,et al."Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus".AQUACULTURE 543(2021):9.

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