An invertebrate gene encoding a Mab21-containing protein involves in antiviral response through regulating the STING pathway

doi:10.1016/j.dci.2021.104101

IOCAS-IR > 实验海洋生物学重点实验室

	An invertebrate gene encoding a Mab21-containing protein involves in antiviral response through regulating the STING pathway
	Li, Shihao 1,2,3; Yang, Feifei 1; Wang, Fuxuan 1; Lv, Xinjia1 ; Li, Fuhua 1,2,3,4
	2021-08-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	121 页码:10
通讯作者	Li, Fuhua([email protected])
摘要	The cGAS-STING pathway plays essential roles in detecting cytosolic dsDNA and initiating antiviral and antibacterial responses in vertebrates. However, knowledge about its function in antiviral response of invertebrates is very limited. In the present study, a gene encoding a Mab21-containing protein, a cGAS homologue, was identified from a decapod crustacean Litopenaeus vannamei and designated as LvMab21cp. LvMab21cp was mainly distributed in intestine and hepatopancreas, showing similar expression profile with other genes in the cGASSTING pathway, such as LvSTING and LvIRF. The expression levels of LvMab21cp, LvSTING and LvIRF were up-regulated in intestine and hepatopancreas of shrimp after white spot syndrome virus (WSSV) infection. Knockdown of LvMab21cp by dsRNA-mediated RNA interference could decrease the expression levels of its putative downstream genes, including LvSTING, LvIRF, LvVago4 and LvVago5, and enhance the in vivo propagation of WSSV in shrimp. Overexpression of LvMab21cp and LvSTING in HEK 293T cells activated the expression of mammalian IFNs upon simulation with interferon stimulatory DNA (ISD). These data suggest that LvMab21cp was a cGAS homologue, a member of the shrimp cGAS-STING pathway, and play an important role during WSSV infection. To our knowledge, this is the first report to show the role of the cGAS-STING pathway in the antiviral response of invertebrates, which will provide new insights into the innate immunity of invertebrates.
关键词	Mab21-containing protein cGAS-STING pathway WSSV Antiviral response Penaeid shrimp
DOI	10.1016/j.dci.2021.104101
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0900502]; General Program of National Natural Science Foundation of China[41776158]; General Program of National Natural Science Foundation of China[31772880]; China Agriculture Research System-48 (CARS-48); China Postdoctoral Science Foundation[2017M612361]; Distinguished Young Scientists Research Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KLEMBDYS01]
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS类目	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS记录号	WOS:000663315700004
出版者	ELSEVIER SCI LTD
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176925
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
第一作者单位	实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心
通讯作者单位	实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Shihao,Yang, Feifei,Wang, Fuxuan,et al. An invertebrate gene encoding a Mab21-containing protein involves in antiviral response through regulating the STING pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,121:10.
APA	Li, Shihao,Yang, Feifei,Wang, Fuxuan,Lv, Xinjia,&Li, Fuhua.(2021).An invertebrate gene encoding a Mab21-containing protein involves in antiviral response through regulating the STING pathway.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,121,10.
MLA	Li, Shihao,et al."An invertebrate gene encoding a Mab21-containing protein involves in antiviral response through regulating the STING pathway".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 121(2021):10.

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