Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals

doi:10.1038/s41467-021-23216-7

IOCAS-IR > 实验海洋生物学重点实验室

	Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals
	Xu, Fei 1,2; Marletaz, Ferdinand 1,3,4; Gavriouchkina, Daria 3,5; Liu, Xiao 6; Sauka-Spengler, Tatjana 5; Zhang, Guofan 2,7; Holland, Peter W. H.1
	2021-05-25
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	12 期号:1 页码:11
通讯作者	Xu, Fei([email protected])
摘要	Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of comparable gene expression patterns is unclear because it is not known if downstream transcriptional targets are also conserved. Here, we report evidence indicating that a classic transcriptional target of Pdx1 in vertebrates, the insulin gene, is a likely direct target of Pdx in Pacific oyster adults. We show that one insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue. Transcriptomic comparison suggests that this tissue plays a similar role to the vertebrate pancreas. Using ATAC-seq and ChIP, we identify an upstream regulatory element of the cgILP gene which shows binding interaction with cgPdx protein in oyster hepatopancreas and demonstrate, using a cell culture assay, that the oyster Pdx can act as a transcriptional activator through this site, possibly in synergy with NeuroD. These data argue that a classic homeodomain-target gene interaction dates back to the origin of Bilateria. In vertebrates insulin is a direct transcriptional target of Pdx: the same is true in Pacific oysters and the authors show insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue, showing this gene interaction dates back to the origin of Bilateria.
DOI	10.1038/s41467-021-23216-7
收录类别	SCI
语种	英语
资助项目	Wellcome Trust[090532/Z/09/Z]; National Natural Science Foundation of China[41776152]; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019Q11]; China Scholarship Council (CSC); European Research Council under the European Union[268513]; Royal Society International Exchanges grant; QR GCRF award
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000658773600012
出版者	NATURE RESEARCH
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176919
专题	实验海洋生物学重点实验室
通讯作者	Xu, Fei
作者单位	1.Univ Oxford, Dept Zool, Oxford, England 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Okinawa Inst Sci & Technol Grad Univ, Mol Genet Unit, Onna, Okinawa, Japan 4.UCL, Ctr Lifes Origins & Evolut, Dept Genet Evolut & Environm, London, England 5.Univ Oxford, Weatherall Inst Mol Med, Radcliffe Dept Med, Oxford, England 6.Zhejiang Ocean Univ, Fishery Coll, Key Lab Marine Fishery Equipment & Technol Zhejia, Zhoushan, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Xu, Fei,Marletaz, Ferdinand,Gavriouchkina, Daria,et al. Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals[J]. NATURE COMMUNICATIONS,2021,12(1):11.
APA	Xu, Fei.,Marletaz, Ferdinand.,Gavriouchkina, Daria.,Liu, Xiao.,Sauka-Spengler, Tatjana.,...&Holland, Peter W. H..(2021).Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals.NATURE COMMUNICATIONS,12(1),11.
MLA	Xu, Fei,et al."Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals".NATURE COMMUNICATIONS 12.1(2021):11.

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