NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments

doi:10.1007/s00343-021-0427-0

	NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments
	Shi, Junfeng 1,3,7; Lei, Yanli1,4,5,6,7 ; Li, Haotian 1,6,7; Li, Tiegang2
	2021-09-01
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
卷号	39 期号:5 页码:1718-1729
通讯作者	Lei, Yanli([email protected]) ; Li, Tiegang([email protected])
摘要	Seamount is a unique deep-sea ecosystem widely distributed in the world. Its biodiversity is vibrant due to its specific geographical and hydrological conditions. However, the diversity and features of foraminifera in such an environment have rarely been studied. We extracted environmental DNA (eDNA) in sediments and amplified the partial small subunit ribosomal DNA (SSU rDNA) of foraminifera to understand the foraminiferal diversity from four sites in Magellan Seamount (Western Pacific Ocean). Partial SSU rDNA sequencing was conducted and 912 979 foraminiferal reads were obtained and gathered into 266 operational taxonomic units (OTUs). In the available dataset, a high proportion of rare OTUs and low identity OTUs in each studied sample showed that the Magellan Seamount foraminiferal community might have a high genetic novelty. The relative abundance of foraminifera varied between replicates probably due to the existence of bias in amplification process and patchiness of the deep-sea floor. It showed that the Magellan Seamount has a relatively high benthic foraminiferal diversity characterized mainly by monothalamiids (76.37% of total reads) in association with rotaliids (19.03% of total reads), including planktic foraminiferal sequences (38.58% of rotaliids; 7.36% of total reads). The remaining reads were assigned to miliolids (0.83% of total reads) and textulariids (0.66% of total reads), and 3.11% of total reads are unassigned to a specific family. The comparative analysis with foraminiferal assemblages from coastal and deep-sea environments indicated that seamounts could aggregate species from a nearby deep-sea.
关键词	metabarcoding Magellan Seamount foraminifera Western Pacific Ocean
DOI	10.1007/s00343-021-0427-0
收录类别	SCI
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000704796400011
出版者	SCIENCE PRESS
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176652
专题	海洋生物分类与系统演化实验室
通讯作者	Lei, Yanli; Li, Tiegang
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 2.Minist Nat Resources MNR, Key Lab Marine Sedimentol & Environm Geol, Inst Oceanog 1, Qingdao 266061, Peoples R China 3.Weifang Med Univ, Clin Res Ctr, Affiliated Hosp, Weifang 261031, Peoples R China 4.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519082, Peoples R China 5.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Shi, Junfeng,Lei, Yanli,Li, Haotian,et al. NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2021,39(5):1718-1729.
APA	Shi, Junfeng,Lei, Yanli,Li, Haotian,&Li, Tiegang.(2021).NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,39(5),1718-1729.
MLA	Shi, Junfeng,et al."NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 39.5(2021):1718-1729.

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