Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5

doi:10.1016/j.ijbiomac.2021.08.159

IOCAS-IR

	Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5
	Wei, Maosheng 1,2,3,4; Geng, Lihua 1,2,3; Wang, Qingchi1,2,3,4 ; Yue, Yang1,2,3 ; Wang, Jing1,2,3 ; Wu, Ning 1,2,3; Wang, Xiaoqing 1,2,3,4; Sun, Chaomin 1,2,3; Zhang, Quanbin 1,2,3
	2021-10-31
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	189 页码:649-656
通讯作者	Geng, Lihua([email protected]) ; Zhang, Quanbin([email protected])
摘要	Crude exopolysaccharides from extracellular polymeric substances produced by the marine bacterium Bacillus sp. H5 were fractionated using DEAE-Sepharose FF and Sephadex G-75 chromatography. The high molecular weight fraction (89.0 kD) from the neutral fraction was designated EPS5SH; it contained mannose, glucosamine, glucose, and galactose in a molar ratio of 1.00: 0.02: 0.07: 0.02. Infra-red, gas chromatography-mass spectrometry, electrospray ionisation-tandem mass spectrometry analysis and nuclear magnetic resonance revealed EPS5SH was a mannan with alpha-(1-* 4)-Manp, alpha-(1-* 2)-Manp, alpha-(1-* 4, 6)-Manp and 8-terminal-Manp. Preliminary in vitro experiments revealed that EPS5SH significantly upregulated nitric oxide synthesis and release of pro inflammatory factors in murine macrophage RAW264.7 cells. Western blot experiments verified the immunostimulatory effects of EPS5SH through the modulation of the NF-kappa B and MAPK signalling pathways. In conclusion, EPS5SH was a novel immunostimulatory mannan.
关键词	Exopolysaccharide Characteristics Immunostimulatory effects
DOI	10.1016/j.ijbiomac.2021.08.159
收录类别	SCI
语种	英语
资助项目	China Ocean Mineral Resources RD Association[DY135-B2-14]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000702873200006
出版者	ELSEVIER
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176530
专题	中国科学院海洋研究所
通讯作者	Geng, Lihua; Zhang, Quanbin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS, Ctr Ocean Megasci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wei, Maosheng,Geng, Lihua,Wang, Qingchi,et al. Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,189:649-656.
APA	Wei, Maosheng.,Geng, Lihua.,Wang, Qingchi.,Yue, Yang.,Wang, Jing.,...&Zhang, Quanbin.(2021).Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,189,649-656.
MLA	Wei, Maosheng,et al."Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 189(2021):649-656.

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