Visual system development and changes in hatching performance in hybrid grouper embryos under different light conditions

doi:10.1016/j.aqrep.2021.100814

IOCAS-IR > 实验海洋生物学重点实验室

	Visual system development and changes in hatching performance in hybrid grouper embryos under different light conditions
	Ren, Liping 1,2,3,4; Wu, Lele3,4,5,6 ; Liu, Feng 3,4,5,6; Song, Yuanzhao 8; Li, Jun 3,4,5; Xu, Shihong 3,4,5; Song, Changbin 7; Wang, Yanfeng 3,4,5; Li, Xian 2,3
	2021-11-01
发表期刊	AQUACULTURE REPORTS
ISSN	2352-5134
卷号	21 页码:8
通讯作者	Li, Xian([email protected])
摘要	Light greatly impacts the performance of teleost embryos. In this study, the development of visual system and performance of Epinehelus moara x Epinephelus lanceolatus embryos under different light spectra (red, orange, green, blue and full spectrum) and dark conditions were assessed. The development of visual system was not completed during embryonic development, characterized by the absence of structural integrity in the retina, indicating the absence of visual photosensitivity. However, nonvisual system may play an important role in photosensitivity. Light conditions contributed significantly to embryo performance, including hatching rate, deformity rate and Wnt signaling pathway expression. There was no statistical difference in the oxygen consumption rate of embryos in different groups during heartbeat onset stage. Embryos in dark group had a significantly lower hatching rate than other groups. The hatching rate in full light spectrum group was significantly higher than red spectrum group, and no significant differences were observed among the other groups. The deformity rate of newly hatched larvae exposed to orange light was significantly lower than those under full spectrum and dark conditions. The mRNA expression level of frizzled2, dickkopf2, limb bud and heart homolog, and natural kermes dye inhibitor of Wnt signaling pathway 1 were detected. The results suggested that dark conditions could lead to the abnormal expression of the Wnt signaling pathway, characterized by significantly lower mRNA expression levels of these factors, which in turn may cause decreased hatchability. In order to increase the hatchability and performance of embryos, and reduce the deformity rate, orange light should be utilized and dark conditions should be avoided during the embryonic development of E. moara x E. lanceolatus.
关键词	Epinehelus moara x Epinephelus lanceolatus Embryo Light condition Performance
DOI	10.1016/j.aqrep.2021.100814
收录类别	SCI
语种	英语
资助项目	Key R&D Program of Guangdong Province[2019B020215001]; National Natural Science Foundation[41976122]; National Key R&D Program of China[2017YFB0404000]; Key R&D Program of Shandong Province[2018GHY115031]; China Agriculture Research System[CARS-47]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:000701782600001
出版者	ELSEVIER
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176498
专题	实验海洋生物学重点实验室
通讯作者	Li, Xian
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 2.Ocean Univ China, Fisheries Coll, Qingdao 266001, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Inst Semicond, Beijing 100083, Peoples R China 8.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Ren, Liping,Wu, Lele,Liu, Feng,et al. Visual system development and changes in hatching performance in hybrid grouper embryos under different light conditions[J]. AQUACULTURE REPORTS,2021,21:8.
APA	Ren, Liping.,Wu, Lele.,Liu, Feng.,Song, Yuanzhao.,Li, Jun.,...&Li, Xian.(2021).Visual system development and changes in hatching performance in hybrid grouper embryos under different light conditions.AQUACULTURE REPORTS,21,8.
MLA	Ren, Liping,et al."Visual system development and changes in hatching performance in hybrid grouper embryos under different light conditions".AQUACULTURE REPORTS 21(2021):8.

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