Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway

doi:10.1016/j.biortech.2021.125788

IOCAS-IR > 实验海洋生物学重点实验室

	Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway
	Yu, Wenjie1,2,3 ; Zhang, Litao 1,2; Zhao, Jing 1,2,4; Liu, Jianguo 1,2
	2021-12-01
发表期刊	BIORESOURCE TECHNOLOGY
ISSN	0960-8524
卷号	341 页码:9
通讯作者	Liu, Jianguo([email protected])
摘要	Improvement of astaxanthin yield is a continuing objective in Haematococcus pluvialis cultivation. In this study, a new strategy combining exogenous sodium fumarate (SF) with nitrogen deficiency was demonstrated to promote astaxanthin accumulation in H. pluvialis. SF significantly accelerated astaxanthin synthesis over cell growth. Notably, under nitrogen deficiency, 10 mM SF increased the cellular astaxanthin content by 1.75-fold and 1.47 fold on day 7 and 12, respectively. Further studies indicated that SF increased the level of respiratory metabolites in the Embden-Meyerhof-Parnas pathway, tricarboxylic acid cycle and pentose phosphate pathway. An enhanced substrate pool due to the acceleration of respiratory metabolic pathways directly induced astaxanthin synthesis. Meanwhile, the accumulation of C18:1, C18:2 and C18:3 fatty acids enhanced astaxanthin synthesis indirectly by promoting the process of esterification. This study not only helps to elucidate the role of respiratory metabolic pathways in astaxanthin synthesis but also provides a new effective technique to improve astaxanthin production.
关键词	Astaxanthin Haematococcus pluvialis Sodium fumarate Respiratory metabolic pathway Nitrogen deficiency
DOI	10.1016/j.biortech.2021.125788
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFE0107200]; National Natural Science Foundation of China[31872588]; National Natural Science Foundation of China[U1706209]; Shandong Provincial Natural Science Foundation, China[ZR2019BC057]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000694798100003
出版者	ELSEVIER SCI LTD
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176419
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yu, Wenjie,Zhang, Litao,Zhao, Jing,et al. Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway[J]. BIORESOURCE TECHNOLOGY,2021,341:9.
APA	Yu, Wenjie,Zhang, Litao,Zhao, Jing,&Liu, Jianguo.(2021).Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway.BIORESOURCE TECHNOLOGY,341,9.
MLA	Yu, Wenjie,et al."Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway".BIORESOURCE TECHNOLOGY 341(2021):9.

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