Different Immune Responses of the Lymphoid Organ in Shrimp at Early Challenge Stage of Vibrio parahaemolyticus and WSSV

doi:10.3390/ani11082160

IOCAS-IR > 实验海洋生物学重点实验室

	Different Immune Responses of the Lymphoid Organ in Shrimp at Early Challenge Stage of Vibrio parahaemolyticus and WSSV
	Wang, Fuxuan1 ; Li, Shihao 1,2,3; Li, Fuhua 1,2,3,4
	2021-08-01
发表期刊	ANIMALS
ISSN	2076-2615
卷号	11 期号:8 页码:15
通讯作者	Li, Shihao([email protected])
摘要	Simple Summary Disease is a frequently encountered problem in aquaculture, which always causes global economic losses. White spot syndrome virus (WSSV) and Vibrio parahaemolyticus are two of the most destructive pathogens causing severe loss of shrimp aquaculture. Understanding the host immune responses against different pathogens is vital for developing effective disease control technologies. The lymphoid organ is a vital part of the shrimp immune system and exhibits important immune functions including cellular and humoral immunity. However, the immune function of the lymphoid organ and its responses against different pathogens are still largely unclear. In the present study, transcriptomic analysis was applied to compare the differentially expressed genes (DEGs) in the lymphoid organ of shrimp after Vibrio or WSSV challenge. Data showed that Vibrio challenge induced broad immune responses in the lymphoid organ including activation of several pattern recognition receptors, the proPO activating system, phagocytosis related genes, and immune effectors. In contrast, the immune responses seemed to be inhibited after WSSV infection. The present study suggests that the shrimp lymphoid organ plays different functions in response to the infection of distinct pathogens at early stage, which provides new insights into the immune functions of lymphoid organ in shrimp. The lymphoid organ is an essential part of the immune system involved in cellular and humoral immune responses in shrimp. However, its roles in the immune responses against different pathogens are still largely unclear. In the present study, transcriptomic analysis was applied to compare the differentially expressed genes (DEGs) in the lymphoid organ of shrimp after Vibrio or WSSV challenge. In total, 2127 DEGs were screened in the lymphoid organ of shrimp at 6 h post Vibrio parahaemolyticus injection, and 1569 DEGs were obtained at the same time after WSSV challenge. KEGG pathway enrichment analysis of these DEGs revealed that two significantly enriched pathways including "neuroactive ligand-receptor interaction" and "protein digestion and absorption" were responsive to both pathogens. In contrast, "lysosome" was the significantly enriched pathway only in Vibrio challenge whereas carbohydrate metabolism related pathways were the significantly enriched pathways only in WSSV challenge. Further analysis on immune-related DEGs showed that Vibrio challenge induced broad immune responses in the lymphoid organ including activation of several pattern recognition receptors, the proPO activating system, phagocytosis related genes, and immune effectors. In contrast, the immune responses seemed to be inhibited after WSSV infection. The data suggest that the shrimp lymphoid organ plays different functions in response to the infection of distinct pathogens at the early stage, which provides new insights into the immune functions of lymphoid organ in shrimp.
关键词	lymphoid organ immune response Vibrio parahaemolyticus WSSV Litopenaeus vannamei
DOI	10.3390/ani11082160
收录类别	SCI
语种	英语
资助项目	Key Program of the National Natural Science Foundation of China[31830100]; General Program of National Natural Science Foundation of China[31972829]; General Program of National Natural Science Foundation of China[41776158]; China Agriculture Research System of MOF; Distinguished Young Scientists Research Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KLEMB-DYS01]
WOS研究方向	Agriculture ; Veterinary Sciences
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS记录号	WOS:000688703800001
出版者	MDPI
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176261
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, Fuxuan,Li, Shihao,Li, Fuhua. Different Immune Responses of the Lymphoid Organ in Shrimp at Early Challenge Stage of Vibrio parahaemolyticus and WSSV[J]. ANIMALS,2021,11(8):15.
APA	Wang, Fuxuan,Li, Shihao,&Li, Fuhua.(2021).Different Immune Responses of the Lymphoid Organ in Shrimp at Early Challenge Stage of Vibrio parahaemolyticus and WSSV.ANIMALS,11(8),15.
MLA	Wang, Fuxuan,et al."Different Immune Responses of the Lymphoid Organ in Shrimp at Early Challenge Stage of Vibrio parahaemolyticus and WSSV".ANIMALS 11.8(2021):15.

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