RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2

doi:10.1111/jpy.13197

IOCAS-IR > 实验海洋生物学重点实验室

	RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2
	He, Bangxiang 1,2,3; Gu, Wenhui 1,2; Wang, Lijun 1,2; Zheng, Zhenbing 1,2; Shao, Zhizhuo 1,2; Huan, Li1,2 ; Zhang, Baoyu 1,2; Ma, Yingchao 1,2; Niu, Jianfeng 1,2; Xie, Xiujun 1,2; Wang, Guangce 1,2
	2021-08-04
发表期刊	JOURNAL OF PHYCOLOGY
ISSN	0022-3646
页码	11
通讯作者	Xie, Xiujun([email protected]) ; Wang, Guangce([email protected])
摘要	In the life cycle of Neopyropia yezoensis, a potential model system for marine macroalgae, both asexual archeospores and meiosis-related conchospores develop into thalli (gametophyte). To understand this special life phenomenon in macroalgae, we picked out the two kinds of spores (10-30 cells in each sample) and conducted RNA-seq using Smart-seq2. Comparative analysis showed that light capture and carbon fixation associated differentially expressed genes (DEGs) were upregulated in archeospores, thus indicating that archeospores are in a state of rapid vegetative growth. In conchospores, protein synthesis and degradation, especially molecular chaperone, associated DEGs were up-regulated, indicating that complex life activities might be occurring in conchospores. There were 68 genes related to DNA replication and repair expressed in conchospores, showing that active DNA replication might occur in conchospores. Moreover, we found that one conchospore specifically expressed DEG (py04595: DNA helicase) only in diploid stages (conchocelis, sporangial filament) and three archeospores specifically expressed DEGs only in haploid stages (thalli). These molecular level results indicated that conchospores were closer to diploid, and might be the meiotic mother cells of N. yezoensis. In addition, we found that the knotted-like homeobox gene (PyKNOX), which might relate to the transition of gametophyte from sporophyte, was only expressed in sporophyte generation but not expressed in conchospores, archeospores and thalli, indicating the morphogenesis of gametophyte sin N. yezoensis might require the inactivation of PyKNOX.
关键词	archeospores asexual reproduction conchospores meiosis RNA-seq Smart-seq2
DOI	10.1111/jpy.13197
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901500]; National Natural Science Foundation of China[41876160]; National Natural Science Foundation of China[41776150]; National Natural Science Foundation of China[41906105]; National Natural Science Foundation of China[41876163]; Key Deployment Project of the Centre for Ocean Mega-Research of Science, the Chinese Academy of Sciences[COMS2019Q02]; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815]; North Jiangsu Science and Technology Special Project[LYG-SZ201916]; Science and Technology Project of Dongtou District[N2016Y18A]
WOS研究方向	Plant Sciences ; Marine & Freshwater Biology
WOS类目	Plant Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000680918000001
出版者	WILEY
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/175945
专题	实验海洋生物学重点实验室
通讯作者	Xie, Xiujun; Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He, Bangxiang,Gu, Wenhui,Wang, Lijun,et al. RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2[J]. JOURNAL OF PHYCOLOGY,2021:11.
APA	He, Bangxiang.,Gu, Wenhui.,Wang, Lijun.,Zheng, Zhenbing.,Shao, Zhizhuo.,...&Wang, Guangce.(2021).RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2.JOURNAL OF PHYCOLOGY,11.
MLA	He, Bangxiang,et al."RNA-seq between asexual archeospores and meiosis-related conchospores in Neopyropia yezoensis using Smart-seq2".JOURNAL OF PHYCOLOGY (2021):11.

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