The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)

doi:10.3389/fmars.2021.690282

	The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)
	Yang, Mei-Jie 1,2,3,4,5; Feng, Jie 1,2,3,4; Song, Hao 1,2,3,4; Yu, Zheng-Lin 1,2,3,4; Shi, Pu1,2,3,4,5 ; Liang, Jian 6; Hu, Zhi 1,2,3,4,5; Zhou, Cong1,2,3,4,5 ; Wang, Xiao-Lin 1,2,3,4; Zhang, Tao1,2,3,4
	2021-07-16
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	8 页码:14
通讯作者	Zhang, Tao([email protected])
摘要	Metamorphosis is a critical developmental event in mollusks, and neuroendocrine system plays an essential role in this process. Rapana venosa is an economically important shellfish in China, but the artificial technology of R. venosa aquaculture is limited by metamorphosis. As a carnivorous gastropod, food habit transition makes the mechanism of R. venosa metamorphosis more complex. To investigate the changes in the neuroendocrine system and to reveal its role in regulating the food habit transition and metamorphosis of R. venosa, we cloned the cDNA sequences encoding 5-hydroxytryptamine receptor (Rv-5HTR), nitric oxide synthetase (Rv-NOS) and cholecystokinin receptor (Rv-CCKR), and investigated their expression by quantitative real-time PCR analysis, and explore the spatio-temporal changes of 5-HT protein expression using Immunohistochemical (IHC) analysis. The expression of the three geens was significantly increased in the early intramembrane veliger stage, which indicates that the three genes are related to the development of digestive system. Additionally, expression of the three genes was decreased after metamorphosis, while Rv-NOS and Rv-CCKR were increasingly expressed in competent larvae, which may help the larvae find suitable environments and promote digestive system development for metamorphosis, and the result of 5-HT IHC analysis also reflects the development of neuroendocrine system. Furthermore, results show that CCK can effect the expression of digestive enzyme, NOS and 5-HT receptor. Finally, based on the present results, we hypothesized that CCK and CCK receptor may be critical regulatory factors of food habit transition and metamorphosis. These results might provide information on the development of neuroendocrine system of R. venosa, and new insight into the regulation of the food habit transition and metamorphosis of gastropods.
关键词	Rapana venosa metamorphosis neuroendocrine system food habit transition digestive system
DOI	10.3389/fmars.2021.690282
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31972814]; National Natural Science Foundation of China[32002409]; Natural Science Foundation of Shandong Province[ZR2019BD003]; China Postdoctoral Science Foundation[2019M652498]; Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2019JZZY020708]; Earmarked Fund for Modern Agro-Industry Technology Research System[CARS-49]; Industry Leading Talents Project of Taishan Scholars[LJNY201704]; Double Hundred Blue Industry Leader Team of Yantai; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEESCTSP2018-1]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000679892100001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/175917
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yang, Mei-Jie,Feng, Jie,Song, Hao,et al. The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)[J]. FRONTIERS IN MARINE SCIENCE,2021,8:14.
APA	Yang, Mei-Jie.,Feng, Jie.,Song, Hao.,Yu, Zheng-Lin.,Shi, Pu.,...&Zhang, Tao.(2021).The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846).FRONTIERS IN MARINE SCIENCE,8,14.
MLA	Yang, Mei-Jie,et al."The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)".FRONTIERS IN MARINE SCIENCE 8(2021):14.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
fmars-08-690282.pdf（4042KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览