Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea

doi:10.3390/ijms22147325

	Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea
	Li, Fengting1,2 ; Yang, Aoao1 ; Hu, Zhangxi1,3,4 ; Lin, Siheng1 ; Deng, Yunyan 1,3,4; Tang, Ying Zhong 1,3,4
	2021-07-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	22 期号:14 页码:17
通讯作者	Deng, Yunyan([email protected]) ; Tang, Ying Zhong([email protected])
摘要	Energetic metabolism is essential in maintaining the viability of all organisms. Resting cysts play important roles in the ecology of dinoflagellates, particularly for harmful algal blooms (HABs)-causative species. However, the energetic metabolism underlying the germination potency maintenance of resting cysts of dinoflagellate have been extremely scarce in studies from physiological and, particularly, molecular perspectives. Therefore, we used the cosmopolitan Scrippsiella trochoidea as a representative of HABs-forming and cyst-producing dinoflagellates in this work to obtain novel insights into the molecular mechanisms, regulating the energetic metabolism in dinoflagellate resting cysts, under different physical condition. As the starting step, we established a cDNA subtractive library via suppression subtractive hybridization (SSH) technology, from which we screened an incomplete sequence for the beta subunit of ATP synthase gene (beta-F-1-ATPase), a key indicator for the status of cell's energetic metabolism. The full-length cDNA of beta-F-1-ATPase gene from S.trochoidea (St beta-F-1-ATPase) was then obtained via rapid amplification of cDNA ends (RACE) (Accession: MZ343333). Our real-time qPCR detections, in vegetative cells and resting cysts treated with different physical conditions, revealed that (1) the expression of St beta-F 1 -ATPase in resting cysts was generally much lower than that in vegetative cells, and (2) the St beta-F-1-ATPase expressions in the resting cysts under darkness, lowered temperature, and anoxia, and during an extended duration of dormancy, were significantly lower than that in cysts under the condition normally used for culture-maintaining (a 12 h light:12 h dark cycle, 21 degrees C, aerobic, and newly harvested). Our detections of the viability (via Neutral Red staining) and cellular ATP content of resting cysts, at the conditions corresponding to the abovementioned treatments, showed that both the viability and ATP content decreased rapidly within 12 h and then maintained at low levels within the 4-day experimentation under all the three conditions applied (4 degrees C, darkness, and anoxia), which are well in accordance with the measurements of the transcription of St beta-F-1-ATPase. These results demonstrated that the energy consumption of resting cysts reaches a low, but somehow stable, level within a short time period and is lower at low temperature, darkness, and anoxia than that at ambient temperature. Our work provides an important basis for explaining that resting cysts survive long-term darkness and low temperature in marine sediments from molecular and physiological levels.
关键词	ATP content ATP synthase subunit beta (beta-F-1-ATPase) energetic metabolism resting cyst Scrippsiella trochoidea suppression subtractive hybridization (SSH) viability
DOI	10.3390/ijms22147325
收录类别	SCI
语种	英语
资助项目	Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q09]; National Science Foundation of China[41606126]; National Science Foundation of China[41776125]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000677344800001
出版者	MDPI
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/175814
专题	海洋生态与环境科学重点实验室
通讯作者	Deng, Yunyan; Tang, Ying Zhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Fengting,Yang, Aoao,Hu, Zhangxi,et al. Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(14):17.
APA	Li, Fengting,Yang, Aoao,Hu, Zhangxi,Lin, Siheng,Deng, Yunyan,&Tang, Ying Zhong.(2021).Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(14),17.
MLA	Li, Fengting,et al."Probing the Energetic Metabolism of Resting Cysts under Different Conditions from Molecular and Physiological Perspectives in the Harmful Algal Blooms-Forming Dinoflagellate Scrippsiella trochoidea".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.14(2021):17.

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