Genome-wide survey of putative serine/threonine protein kinases in cyanobacteria

doi:10.1186/1471-2164-8-395

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	Genome-wide survey of putative serine/threonine protein kinases in cyanobacteria
	Zhang, Xiaowen 1,2; Zhao, Fangqing 1,2; Guan, Xiangyu 3; Yang, Yu 1,2; Liang, Chengwei 1,2; Qin, Song 1; Qin, S, Chinese Acad Sci, Inst Oceanol, Nanhai Rd, Qingdao, Peoples R China
	2007-10-30
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	8 页码:395
文章类型	Article
摘要	Background: Serine/threonine kinases (STKs) have been found in an increasing number of prokaryotes, showing important roles in signal transduction that supplement the well known role of two-component system. Cyanobacteria are photoautotrophic prokaryotes able to grow in a wide range of ecological environments, and their signal transduction systems are important in adaptation to the environment. Sequence information from several cyanobacterial genomes offers a unique opportunity to conduct a comprehensive comparative analysis of this kinase family. In this study, we extracted information regarding Ser/Thr kinases from 21 species of sequenced cyanobacteria and investigated their diversity, conservation, domain structure, and evolution.; Background: Serine/threonine kinases (STKs) have been found in an increasing number of prokaryotes, showing important roles in signal transduction that supplement the well known role of two-component system. Cyanobacteria are photoautotrophic prokaryotes able to grow in a wide range of ecological environments, and their signal transduction systems are important in adaptation to the environment. Sequence information from several cyanobacterial genomes offers a unique opportunity to conduct a comprehensive comparative analysis of this kinase family. In this study, we extracted information regarding Ser/Thr kinases from 21 species of sequenced cyanobacteria and investigated their diversity, conservation, domain structure, and evolution. Results: 286 putative STK homologues were identified. STKs are absent in four Prochlorococcus strains and one marine Synechococcus strain and abundant in filamentous nitrogen-fixing cyanobacteria. Motifs and invariant amino acids typical in eukaryotic STKs were conserved well in these proteins, and six more cyanobacteria- or bacteria-specific conserved residues were found. These STK proteins were classified into three major families according to their domain structures. Fourteen types and a total of 131 additional domains were identified, some of which are reported to participate in the recognition of signals or substrates. Cyanobacterial STKs show rather complicated phylogenetic relationships that correspond poorly with phylogenies based on 16S rRNA and those based on additional domains. Conclusion: The number of STK genes in different cyanobacteria is the result of the genome size, ecophysiology, and physiological properties of the organism. Similar conserved motifs and amino acids indicate that cyanobacterial STKs make use of a similar catalytic mechanism as eukaryotic STKs. Gene gain-and-loss is significant during STK evolution, along with domain shuffling and insertion. This study has established an overall framework of sequence-structure-function interactions for the STK gene family, which may facilitate further studies of the role of STKs in various organisms.
关键词	Sp Strain Pcc-7120 Synechocystis Sp Pcc-6803 Signal-transduction Sequence Alignment Catalytic Domain Sensory Domains Ser/thr Kinases Gaf Domain Fha Domain Bacteria
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/1471-2164-8-395
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000252440000001
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1739
专题	实验海洋生物学重点实验室
通讯作者	Qin, S, Chinese Acad Sci, Inst Oceanol, Nanhai Rd, Qingdao, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Ocean Univ China, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Xiaowen,Zhao, Fangqing,Guan, Xiangyu,et al. Genome-wide survey of putative serine/threonine protein kinases in cyanobacteria[J]. BMC GENOMICS,2007,8:395.
APA	Zhang, Xiaowen.,Zhao, Fangqing.,Guan, Xiangyu.,Yang, Yu.,Liang, Chengwei.,...&Qin, S, Chinese Acad Sci, Inst Oceanol, Nanhai Rd, Qingdao, Peoples R China.(2007).Genome-wide survey of putative serine/threonine protein kinases in cyanobacteria.BMC GENOMICS,8,395.
MLA	Zhang, Xiaowen,et al."Genome-wide survey of putative serine/threonine protein kinases in cyanobacteria".BMC GENOMICS 8(2007):395.

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