Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong

doi:10.1111/j.1574-6941.2007.00353.x

IOCAS-IR > 实验海洋生物学重点实验室

	Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong
	Zhang, Rui; Liu, Baozhong; Lau, Stanley C. K.; Ki, Jang-Seu ; Qian, Pei-Yuan ; Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China
	2007-09-01
发表期刊	FEMS MICROBIOLOGY ECOLOGY
ISSN	0168-6496
卷号	61 期号:3 页码:496-508
文章类型	Article
摘要	Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed.; Diversity of particle-attached and free-living marine bacteria in Victoria Harbor, Hong Kong, and its adjacent coastal and estuarial environments was investigated using DNA fingerprinting and clone library analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes showed that bacterial communities in three stations of Victoria Harbor were similar, but differed from those in adjacent coastal and estuarine stations. Particle-attached and free-living bacterial community composition differed in the Victoria Harbor area. DNA sequencing of 28 bands from DGGE gel showed Alphaproteobacteria was the most abundant group, followed by the Bacteroidetes, and other Proteobacteria. Bacterial species richness (number of DGGE bands) differed among stations and populations (particle-attached and free-living; bottom and surface). BIOENV analysis indicated that the concentrations of suspended solids were the major contributing parameter for the spatial variation of total bacterial community structure. Samples from representative stations were selected for clone library (548 clones) construction and their phylogenetic distributions were similar to those of sequences from DGGE. Approximately 80% of clones were affiliated to Proteobacteria, Bacteroidetes and Cyanobacteria. The possible influences of dynamic pollution and hydrological conditions in the Victoria Harbor area on the particle-attached and free-living bacterial community structures were discussed.
关键词	Particle-attached Free-living Contrasting Marine Environment
学科领域	Microbiology
DOI	10.1111/j.1574-6941.2007.00353.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000248961900010
引用统计	被引频次：108[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1725
专题	实验海洋生物学重点实验室
通讯作者	Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China
作者单位	1.Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Rui,Liu, Baozhong,Lau, Stanley C. K.,et al. Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong[J]. FEMS MICROBIOLOGY ECOLOGY,2007,61(3):496-508.
APA	Zhang, Rui,Liu, Baozhong,Lau, Stanley C. K.,Ki, Jang-Seu,Qian, Pei-Yuan,&Qian, PY, Hong Kong Univ Sci & Technol, Dept Biol & Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China.(2007).Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong.FEMS MICROBIOLOGY ECOLOGY,61(3),496-508.
MLA	Zhang, Rui,et al."Particle-attached and free-living bacterial communities in a contrasting marine environment: Victoria Harbor, Hong Kong".FEMS MICROBIOLOGY ECOLOGY 61.3(2007):496-508.

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