Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform
Song, Fengge1; Wei, Yangdao1; Wang, Peng3; Ge, Xiaolin1; Li, Chaoyang1; Wang, Aimin1; Yang, Zhiqing1; Wan, Yi1,3,4; Li, Jinghong2
2021-08-01
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号185页码:5
通讯作者Yang, Zhiqing([email protected]) ; Wan, Yi([email protected])
摘要Nucleic acid-based diagnosis using CRISPR-Cas associated enzymes is essential for rapid infectious disease diagnosis and treatment strategies during a global pandemic. The obstacle has been blossomed CRIPSR-Cas based tools that can monitor wide range of pathogens in clinical samples with ultralow concentrations. Here, a universal nucleic acid magneto-DNA nanoparticle system was exploited for the detection of pathogenic bacteria, based on the collateral cleavage activity of CRISPR-Cas14a and tag-specific primer extension. In the system, the target nucleic acids were amplificated and be separated from mixtures by streptavidin-coated magnetic bead. The collateral cleavage activity of CRISPR-Cas14a can be activated via the tag sequence on the target product. Consequently, the fluorophore quencher reporter can be activated by CRISPR-Cas14a, leading to the increasing response. The exploited universal bacterial diagnostic can distinguish six different bacteria strains with 1 cfu/mL or 1 aM sensitivity, which may provide new strategies to construct fast, accurate, cost-effective and sensitive diagnostic tools in environments with limited resources.
关键词Bacterial diagnostics CRISPR-Cas14a Tag-specific primer extension Magneto-DNA
DOI10.1016/j.bios.2021.113262
收录类别SCI
语种英语
资助项目Hainan Provincial Natural Science Foundation of China[420RC518]; Open Fund of Shandong Key Laboratory of Corrosion Science[KLCS201910]; Tsinghua University Initiative Scientific Research Program[2020Z99CFZ019]; Tsinghua University Spring Breeze Fund[2020Z99CFZ019]; Research Foundation of Hainan University[KYQD (ZR) 1711]; Research Foundation of Hainan University[KYQD (ZR) 1997]; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF04]; Open fund of Fujian Provincial Key Laboratory of Photonics Technology[JYG 2002]
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号WOS:000655570200001
出版者ELSEVIER ADVANCED TECHNOLOGY
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被引频次:45[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/171002
专题海洋环境腐蚀与生物污损重点实验室
通讯作者Yang, Zhiqing; Wan, Yi
作者单位1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China
2.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Bio Fouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
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Song, Fengge,Wei, Yangdao,Wang, Peng,et al. Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform[J]. BIOSENSORS & BIOELECTRONICS,2021,185:5.
APA Song, Fengge.,Wei, Yangdao.,Wang, Peng.,Ge, Xiaolin.,Li, Chaoyang.,...&Li, Jinghong.(2021).Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform.BIOSENSORS & BIOELECTRONICS,185,5.
MLA Song, Fengge,et al."Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform".BIOSENSORS & BIOELECTRONICS 185(2021):5.
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