A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities

doi:10.3390/md19050250

IOCAS-IR > 实验海洋生物学重点实验室

	A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities
	Sun, Mingzhe1 ; Li, Shihao 1,2,3; Lv, Xinjia1 ; Xiang, Jianhai1,2,3 ; Lu, Yuanan 4; Li, Fuhua 1,2,3,5
	2021-05-01
发表期刊	MARINE DRUGS
卷号	19 期号:5 页码:15
通讯作者	Li, Shihao([email protected]) ; Li, Fuhua([email protected])
摘要	Different shrimp species are known to possess apparent distinct resistance to different pathogens in aquaculture. However, the molecular mechanism underlying this finding still remains unknown. One kind of important antimicrobial peptides, anti-lipopolysaccharide factors (ALF), exhibit broad-spectrum antimicrobial activities. Here, we reported a newly identified ALF from the shrimp Litopenaeus vannamei and compared the immune function with its counterpart in the shrimp Fenneropenaeus chinensis. The ALF, designated as LvALF8, was specifically expressed in the lymphoid organ of L. vannamei. The expression level of LvALF8 was apparently changed after white spot syndrome virus (WSSV) or Vibrio parahaemolyticus challenges. The synthetic LBD peptide of LvALF8 (LvALF8-LBD) showed strong antibacterial activities against most tested Gram-negative and Gram-positive bacteria. LvALF8-LBD could also inhibit the in vivo propagation of WSSV similar as FcALF8-LBD, the LBD of LvALF8 counterpart in F. chinensis. However, LvALF8-LBD and FcALF8-LBD exhibited apparently different antibacterial activity against V. parahaemolyticus, the main pathogen causing acute hepatopancreatic necrosis disease (AHPND) of affected shrimp. A structural analysis showed that the positive net charge and amphipathicity characteristics of LvALF8-LBD peptide were speculated as two important components for its enhanced antimicrobial activity compared to those of FcALF8-LBD. These new findings may not only provide some evidence to explain the distinct disease resistance among different shrimp species, but also lay out new research ground for the testing and development of LBD-originated antimicrobial peptides to control of shrimp diseases.
关键词	anti-lipopolysaccharide factor LPS-binding domain lymphoid organ white spot syndrome virus Vibro parahaemolyticus
DOI	10.3390/md19050250
收录类别	SCI
语种	英语
资助项目	Key Program of National Natural Science Foundation of China[31830100]; General Program of National Natural Science Foundation of China[31772880]; General Program of National Natural Science Foundation of China[41776158]; China Agriculture Research system-48 (CARS-48); Distinguished Young Scientists Research Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KLEMB-DYS01]
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000654297500001
出版者	MDPI
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170947
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Hawaii Manoa, Dept Publ Hlth Sci, Environm Hlth Lab, Honolulu, HI 96822 USA 5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Mingzhe,Li, Shihao,Lv, Xinjia,et al. A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities[J]. MARINE DRUGS,2021,19(5):15.
APA	Sun, Mingzhe,Li, Shihao,Lv, Xinjia,Xiang, Jianhai,Lu, Yuanan,&Li, Fuhua.(2021).A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities.MARINE DRUGS,19(5),15.
MLA	Sun, Mingzhe,et al."A Lymphoid Organ Specific Anti-Lipopolysaccharide Factor from Litopenaeus vannamei Exhibits Strong Antimicrobial Activities".MARINE DRUGS 19.5(2021):15.

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