Definition of a High-Resolution Molecular Marker for Tracking the Genetic Diversity of the Harmful Algal Species Eucampia zodiacus Through Comparative Analysis of Mitochondrial Genomes

doi:10.3389/fmicb.2021.631144

	Definition of a High-Resolution Molecular Marker for Tracking the Genetic Diversity of the Harmful Algal Species Eucampia zodiacus Through Comparative Analysis of Mitochondrial Genomes
	Zhang, Mengjia1,2,3,4 ; Cui, Zongmei 1,2,3,4; Liu, Feng 1,2,4; Chen, Nansheng 1,2,4,5
	2021-03-24
发表期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
卷号	12 页码:16
通讯作者	Chen, Nansheng([email protected])
摘要	The cosmopolitan phytoplankton species Eucampia zodiacus is a common harmful algal bloom (HAB) species that have been found to cause HABs in essentially all coastal regions except the Polar regions. However, molecular information for this HAB species is limited with only a few molecular markers. In this project, we constructed the mitochondrial genome (mtDNA) of E. zodiacus, which was also the first mtDNA constructed for any species in the order Hemiaulales that includes 145 reported species (including two additional HAB species Cerataulina bicornis and Cerataulina pelagica). Comparative analysis of eight E. zodiacus strains revealed that they could not be distinguished using common molecular markers, suggesting that common molecular markers do not have adequate resolution for distinguishing E. zodiacus strains. However, these E. zodiacus strains could be distinguished using whole mtDNAs, suggesting the presence of different genotypes due to evolutionary divergence. Through comparative analysis of the mtDNAs of multiple E. zodiacus strains, we identified a new molecular marker ezmt1 that could adequately distinguish different E. zodiacus strains isolated in various coastal regions in China. This molecular marker ezmt1, which was similar to 400 bp in size, could be applied to identify causative genotypes during E. zodiacus HABs through tracking the dynamic changes of genetic diversity of E. zodiacus in HABs.
关键词	harmful algal bloom species Eucampia zodiacus mitochondrial genome genetic marker comparative genomics
DOI	10.3389/fmicb.2021.631144
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000]; Chinese Academy of Sciences Pioneer Hundred Talents Program; Taishan Scholar Project Special Fund; Qingdao Innovation and Creation Plan (Talent Development Program-5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16-zhc]; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023]; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY020706]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000637213500001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170468
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Inst Oceanol, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC, Canada
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Mengjia,Cui, Zongmei,Liu, Feng,et al. Definition of a High-Resolution Molecular Marker for Tracking the Genetic Diversity of the Harmful Algal Species Eucampia zodiacus Through Comparative Analysis of Mitochondrial Genomes[J]. FRONTIERS IN MICROBIOLOGY,2021,12:16.
APA	Zhang, Mengjia,Cui, Zongmei,Liu, Feng,&Chen, Nansheng.(2021).Definition of a High-Resolution Molecular Marker for Tracking the Genetic Diversity of the Harmful Algal Species Eucampia zodiacus Through Comparative Analysis of Mitochondrial Genomes.FRONTIERS IN MICROBIOLOGY,12,16.
MLA	Zhang, Mengjia,et al."Definition of a High-Resolution Molecular Marker for Tracking the Genetic Diversity of the Harmful Algal Species Eucampia zodiacus Through Comparative Analysis of Mitochondrial Genomes".FRONTIERS IN MICROBIOLOGY 12(2021):16.

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