MiR-92 Family Members Form a Cluster Required for Notochord Tubulogenesis in Urochordate Ciona savignyi

doi:10.3390/genes12030406

IOCAS-IR > 实验海洋生物学重点实验室

	MiR-92 Family Members Form a Cluster Required for Notochord Tubulogenesis in Urochordate Ciona savignyi
	Yang, Libo 1; Zhang, Xiaoming 1,2; Liu, Chengzhang3 ; Zhang, Jin 1; Dong, Bo 1,2,4
	2021-03-01
发表期刊	GENES
卷号	12 期号:3 页码:15
通讯作者	Dong, Bo([email protected])
摘要	MicroRNAs are frequently clustered in the genome and polycistronically transcribed, regulating targeted genes in diverse signaling pathways. The miR-17-92 cluster is a typical miRNA cluster, playing crucial roles in the organogenesis and homeostasis of physiological processes in vertebrates. Here, we identified three miRNAs (csa-miR-92a, csa-miR-92b, and csa-miR-92c) that belonged to the miR-92 family and formed a miRNA cluster in the genome of a urochordate marine ascidian Ciona savignyi. Except for miR-92a and miR-92b, other homologs of the vertebrate miR-17-92 cluster members could not be identified in the Ciona genome. We further found that the mature sequences of urochordate miR-92 family members were highly conserved compared with the vertebrate species. The expression pattern revealed that three miR-92 family members had consistent expression levels in adult tissues and were predominantly expressed in heart and muscle tissue. We further showed that, at the embryonic and larval stages, csa-miR-92c was expressed in the notochord of embryos during 18-31 h post fertilization (hpf) by in situ hybridization. Knockout of csa-miR-92c resulted in the disorganization of notochord cells and the block of lumen coalescence in the notochord. Fibroblast growth factor (FGF), mitogen-activated protein kinase (MAPK), and wingless/integrated (Wnt)/planar cell polarity (PCP) signaling pathways might be involved in the regulatory processes, since a large number of core genes of these pathways were the predicted target genes of the miR-92 family. Taken together, we identified a miR-92 cluster in urochordate Ciona and revealed the expression patterns and the regulatory roles of its members in organogenesis. Our results provide expression and phylogenetic data on the understanding of the miR-92 miRNA cluster's function during evolution.
关键词	miRNA cluster miR-92 Ciona notochord lumen formation
DOI	10.3390/genes12030406
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFE0190900]; National Natural Science Foundation of China[41706153]; National Natural Science Foundation of China[31771649]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000633686400001
出版者	MDPI
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170336
专题	实验海洋生物学重点实验室
通讯作者	Dong, Bo
作者单位	1.Ocean Univ China, Sars Fang Ctr, MoE Key Lab Marine Genet & Breeding, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Yang, Libo,Zhang, Xiaoming,Liu, Chengzhang,et al. MiR-92 Family Members Form a Cluster Required for Notochord Tubulogenesis in Urochordate Ciona savignyi[J]. GENES,2021,12(3):15.
APA	Yang, Libo,Zhang, Xiaoming,Liu, Chengzhang,Zhang, Jin,&Dong, Bo.(2021).MiR-92 Family Members Form a Cluster Required for Notochord Tubulogenesis in Urochordate Ciona savignyi.GENES,12(3),15.
MLA	Yang, Libo,et al."MiR-92 Family Members Form a Cluster Required for Notochord Tubulogenesis in Urochordate Ciona savignyi".GENES 12.3(2021):15.

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