Discriminative intracellular and extracellular ATP detection based on magnetically controlled antimicrobial peptide

doi:10.1016/j.snb.2021.129609

	Discriminative intracellular and extracellular ATP detection based on magnetically controlled antimicrobial peptide
	Wang, Yingwen 1,2,3,4; Zhang, Dun 1,2,3; Zeng, Yan1,2,3 ; Ye, Xiangyi1,2,3 ; Sun, Yan 1,2,3; Zhou, Weimin 5; Qi, Peng1,2,3
	2021-05-01
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	334 页码:10
通讯作者	Zhang, Dun([email protected]) ; Qi, Peng([email protected])
摘要	Extracellular free adenosine triphosphate (ATP) is often encountered as an interferent in the detection of intracellular ATP, especially in biofilm systems. To resolve this problem, in this study, a novel impedimetric aptasensor was designed for the discriminative detection of intracellular and extracellular ATP using antimicrobial peptide-functionalized magnetic particles (M-AMPs). Successive fabrication of reduced graphene sheets (rGSs) and gold nano-clusters (Au NCs) on a glassy carbon electrode was conducted by simple electrodeposition methods to achieve improved detection sensitivity. Single-stranded aptamers were attached to the surface of Au NCs for selective ATP binding, and electrochemical impedance spectroscopy was conducted to analyze the change of surface state induced by ATP binding. For discriminative intracellular and extracellular ATP detection, magnetically controlled M-AMPs were introduced into the detection system for mild and rapid bacterial disintegration. In bacterial samples, the M-AMPs were firstly constrained by a magnetic field, and extracellular ATP was selectively bound to the prepared aptasensor, leading to increased impedance response. Thereafter, the magnetic field was shut down to liberate the M-AMPs for cell disruption, and the released intracellular ATP was determined by the further impedance increasement. The results demonstrated that the proposed aptasensor exhibited excellent sensitivity and selectivity, with a wide linear range from 1 nM to 100 nM and a detection limit of 0.65 nM. Remarkably, this electrochemical aptasensor provided a convenient and label-free approach for discriminative intracellular and extracellular ATP detection, and the detection reliability was verified in various bacterial samples and E. coli biofilms.
关键词	ATP detection Intracellular Extracellular Aptasensor Antimicrobial peptides
DOI	10.1016/j.snb.2021.129609
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876101]; National Natural Science Foundation of China[41906037]; Nantong Scientific Plan Foundation[2020NT04]
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000632945700003
出版者	ELSEVIER SCIENCE SA
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170321
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhang, Dun; Qi, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 5.Univ Sci & Technol Liaoning, Key Lab Energy Mat & Electrochem Res Liaoning Pro, Anshan 114051, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Yingwen,Zhang, Dun,Zeng, Yan,et al. Discriminative intracellular and extracellular ATP detection based on magnetically controlled antimicrobial peptide[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,334:10.
APA	Wang, Yingwen.,Zhang, Dun.,Zeng, Yan.,Ye, Xiangyi.,Sun, Yan.,...&Qi, Peng.(2021).Discriminative intracellular and extracellular ATP detection based on magnetically controlled antimicrobial peptide.SENSORS AND ACTUATORS B-CHEMICAL,334,10.
MLA	Wang, Yingwen,et al."Discriminative intracellular and extracellular ATP detection based on magnetically controlled antimicrobial peptide".SENSORS AND ACTUATORS B-CHEMICAL 334(2021):10.

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