C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian

doi:10.1016/j.carbpol.2021.117821

IOCAS-IR > 实验海洋生物学重点实验室

	C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian
	Ma, Yuzhen 1,2,3; Gao, Kun 1,2,3,4; Yu, Huahua1,2,3 ; Liu, Weixiang 1,2,3; Qin, Yukun1,2,3 ; Xing, Ronge1,2,3 ; Liu, Song 1,2,3; Li, Pengcheng1,2,3
	2021-06-01
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	261 页码:13
通讯作者	Yu, Huahua([email protected]) ; Li, Pengcheng([email protected])
摘要	Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases. Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu). FITC-labeled O-CSLn-Cu (FITC-O-CSLn-Cu) was first synthesized via a nucleophilic substitution reaction and confirmed by FT-IR. FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence. O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM). Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by OCSLn-Cu with a particular interest in membrane physicochemical properties. Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated. Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated. Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity. Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.
关键词	Natural organic-based copper O-CSLn-Cu Phytophthora capsici Leonian Membrane damage and structural rearrangement Antifungal mechanism
DOI	10.1016/j.carbpol.2021.117821
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFC0311300]; National Key R&D Program of China[2019YFD0900705]; National Natural Science Foundation of China[42076126]
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000634108100006
出版者	ELSEVIER SCI LTD
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170318
专题	实验海洋生物学重点实验室
通讯作者	Yu, Huahua; Li, Pengcheng
作者单位	1.Chinese Acad Sci, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Ma, Yuzhen,Gao, Kun,Yu, Huahua,et al. C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian[J]. CARBOHYDRATE POLYMERS,2021,261:13.
APA	Ma, Yuzhen.,Gao, Kun.,Yu, Huahua.,Liu, Weixiang.,Qin, Yukun.,...&Li, Pengcheng.(2021).C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.CARBOHYDRATE POLYMERS,261,13.
MLA	Ma, Yuzhen,et al."C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian".CARBOHYDRATE POLYMERS 261(2021):13.

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