A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism

doi:10.3390/md19030176

IOCAS-IR > 实验海洋生物学重点实验室

	A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism
	Wang, Yujian1,2,3,4 ; Zhang, Jian 1,2,5; Sun, Yuanyuan1,2,3 ; Sun, Li 1,2,3,4
	2021-03-01
发表期刊	MARINE DRUGS
卷号	19 期号:3 页码:15
通讯作者	Sun, Li([email protected])
摘要	Crustin is a type of antimicrobial peptide and plays an important role in the innate immunity of arthropods. We report here the identification and characterization of a crustin (named Crus1) from the shrimp Rimicaris sp. inhabiting the deep-sea hydrothermal vent in Manus Basin (Papua New Guinea). Crus1 shares the highest identity (51.76%) with a Type I crustin of Penaeus vannamei and possesses a whey acidic protein (WAP) domain, which contains eight cysteine residues that form the conserved 'four-disulfide core' structure. Recombinant Crus1 (rCrus1) bound to peptidoglycan and lipoteichoic acid, and effectively killed Gram-positive bacteria in a manner that was dependent on pH, temperature, and disulfide linkage. rCrus1 induced membrane leakage and structure damage in the target bacteria, but had no effect on bacterial protoplasts. Serine substitution of each of the 8 Cys residues in the WAP domain did not affect the bacterial binding capacity but completely abolished the bactericidal activity of rCrus1. These results provide new insights into the characteristic and mechanism of the antimicrobial activity of deep sea crustins.
关键词	crustin antimicrobial peptides shrimp deep-sea hydrothermal vent
DOI	10.3390/md19030176
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403]; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309]; Taishan Scholar Program of Shandong Province
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000633841100001
出版者	MDPI
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170294
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China 5.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, Yujian,Zhang, Jian,Sun, Yuanyuan,et al. A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism[J]. MARINE DRUGS,2021,19(3):15.
APA	Wang, Yujian,Zhang, Jian,Sun, Yuanyuan,&Sun, Li.(2021).A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism.MARINE DRUGS,19(3),15.
MLA	Wang, Yujian,et al."A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism".MARINE DRUGS 19.3(2021):15.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
marinedrugs-19-00176（3151KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览