Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities

doi:10.1016/j.chemosphere.2020.129164

IOCAS-IR > 实验海洋生物学重点实验室

	Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities
	Yu, Chunlin1,3 ; Yue, Yang1 ; Yin, Xiujing 1,3; Li, Rongfeng 1,2; Yu, Huahua1,2 ; Li, Pengcheng1,2
	2021-03-01
发表期刊	CHEMOSPHERE
ISSN	0045-6535
卷号	266 页码:13
通讯作者	Yu, Huahua([email protected]) ; Li, Pengcheng()
摘要	Venom geographical variation is common among venomous animals. This phenomenon presents problems in the development of clinical treatments and medicines against envenomation. The venomous giant jellyfish Nemopilema nomurai, Scyphozoan, is a blooming jellyfish species in the Yellow Sea and the East China Sea that causes numerous jellyfish sting cases every year. Metalloprotease and phospholipase A(2) (PLA(2)) are the main components in Nemopilema nomurai venom and may activate many toxicities, such as hemolysis, inflammation and lethality. Geographical variation in the content and activity of these enzymes may cause different symptoms and therapeutic problems. For the first time, we verified metalloprotease and PLA(2) geographical variation in Nemopilema nomurai venom by performing a comparative analysis of 31 venom samples by SDS-PAGE, analyzing protease zymography, enzymatic activity, and drawing contour maps. Band locations and intensities of SDS-PAGE and protease zymograms showed geographical differences. The enzymatic activities of both metalloprotease and PLA(2) showed a trend of geographic regularity. The distribution patterns of these activities are directly shown in contour maps. Metalloproteinase activity was lower near the coast. PLA(2)-like activity was lower in the Southern Yellow Sea. We surmised that metalloproteinase and PLA(2)-like activities might be related to venom ontogeny and species abundance respectively, and influenced by similar environmental factors. This study provides a theoretical basis for further ecological and medical studies of Nemopilema nomurai jellyfish venom. (C) 2020 Elsevier Ltd. All rights reserved.
关键词	Jellyfish Nemopilema nomurai Metalloprotease Phospholipase A(2) Geographical variation
DOI	10.1016/j.chemosphere.2020.129164
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2019YFC0312605]; National Key R&D Program of China[2017YFE0111100-04]; National Natural Science Foundation of China[41776163]; National Natural Science Foundation of China[41876164]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000605756800106
出版者	PERGAMON-ELSEVIER SCIENCE LTD
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170076
专题	实验海洋生物学重点实验室
通讯作者	Yu, Huahua; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yu, Chunlin,Yue, Yang,Yin, Xiujing,et al. Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities[J]. CHEMOSPHERE,2021,266:13.
APA	Yu, Chunlin,Yue, Yang,Yin, Xiujing,Li, Rongfeng,Yu, Huahua,&Li, Pengcheng.(2021).Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities.CHEMOSPHERE,266,13.
MLA	Yu, Chunlin,et al."Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities".CHEMOSPHERE 266(2021):13.

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