Both of marine fish species Oryzias melastigma and Pagrus major all failing in early localization at embryo stage by vasa RNA

doi:10.1016/j.gene.2020.145204

IOCAS-IR > 实验海洋生物学重点实验室

	Both of marine fish species Oryzias melastigma and Pagrus major all failing in early localization at embryo stage by vasa RNA
	Zhou, Li 1,2,3; Xu, Shihong 1,2; Lin, Fan 4; Wang, Xueying1,2 ; Wang, Yunong 1,2,3; Wang, Yanfeng 1,2; Yu, Daode 5; Liu, Qinghua 1,2; Li, Jun 1,2
	2021-02-15
发表期刊	GENE
ISSN	0378-1119
卷号	769 页码:10
通讯作者	Liu, Qinghua([email protected]) ; Li, Jun([email protected])
摘要	Germ cells are essential for gonadal development. As precursors of germ cells, primordial germ cells (PGCs) are particularly important for germline formation. However, the research on distribution patterns of PGCs in marine fish is very limited, especially for economic species. The vasa gene has been widely used as marker to identify PGCs origination and migration because of vasa RNA is a component of germ plasm. In this study, we isolated full-length vasa cDNA (Omvas and Pmvas) from marine medaka (Oryzias melastigma) and red seabream (Pagrus major), detected vasa transcripts in different tissues by RT-PCR and described vasa expression patterns during embryogenesis and gametogenesis by in situ hybridization. At the same time, we also explored the relationship between early distribution of germ plasm components and species evolution. The results demonstrated that deduced amino acid sequence of Omvas and Pmvas shared several conserved motifs of Vasa homologues and high identity with other teleost, and vasa transcripts were exclusively detected in early germ cells of gonad. During embryogenesis, vasa RNA of both fishes, like medaka (Oryzias latipes), failed to localize at cleavage furrows and distributed uniformly throughout each blastomere. This study firstly discovered that the marine economic fish, red seabream, lost vasa RNA early specific localization at cleavage furrows and distinctive distribution in germ cells. In addition, compared with other teleost, we found that early distribution of germ plasm might not relate to species evolution. This will improve our understanding of vasa localization modes in teleost, and facilitate fish germ cell manipulation.
关键词	vasa Marine medaka Red seabream Germ cell Localization
DOI	10.1016/j.gene.2020.145204
收录类别	SCI
语种	英语
资助项目	Shandong Province Key RD projects[2019GHY112013]; National Infrastructure of Fishery Germplasm Resource[2019DKA30470]; National Key Research and Development Program[2018YFD0901205]; National Key Research and Development Program[2018YFD0901204]; National Natural Science Foundation of China[31572602]; China Agriculture Research System[CARS-47]; STS project[KFZD-SW-106]; STS project[2017T3017]; Shandong Province Key Research and Invention Program[2017CXGC010K]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000608240700003
出版者	ELSEVIER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170061
专题	实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shantou Univ, Inst Marine Sci, Guangdong Prov Key Lab Marine Biotechnol, Shantou 515063, Peoples R China 5.Marine Biol Inst Shandong Prov, Qingdao 266104, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhou, Li,Xu, Shihong,Lin, Fan,et al. Both of marine fish species Oryzias melastigma and Pagrus major all failing in early localization at embryo stage by vasa RNA[J]. GENE,2021,769:10.
APA	Zhou, Li.,Xu, Shihong.,Lin, Fan.,Wang, Xueying.,Wang, Yunong.,...&Li, Jun.(2021).Both of marine fish species Oryzias melastigma and Pagrus major all failing in early localization at embryo stage by vasa RNA.GENE,769,10.
MLA	Zhou, Li,et al."Both of marine fish species Oryzias melastigma and Pagrus major all failing in early localization at embryo stage by vasa RNA".GENE 769(2021):10.

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