Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera

doi:10.1007/s10709-020-00112-4

	Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera
	Wang, Yu 1; Liu, Feng 1,2,3; Liu, Manman 1; Shi, Shitao 1; Bi, Yuping 4; Chen, Nansheng 1,2,3
	2021-01-15
发表期刊	GENETICA
ISSN	0016-6707
页码	10
通讯作者	Liu, Feng([email protected])
摘要	Ulva prolifera O.F. Muller (Ulvophyceae, Chlorophyta) is well known as a typical green-tide forming macroalga which has caused the world's largest macroalgal blooms in the Yellow Sea of China. In this study, two full-length gamma-carbonic anhydrase (gamma-CA) genes (Up gamma CA1 and Up gamma CA2) were cloned from U. prolifera. Up gamma CA1 has three conserved histidine residues, which act as an active site for binding a zinc metal ion. In Up gamma CA2, two of the three histidine residues were replaced by serine and arginine, respectively. The two gamma-CA genes are clustered together with other gamma-CAs in Chlorophyta with strong support value (100% bootstrap) in maximum likelihood (ML) phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analysis showed that stressful environmental conditions markedly inhibited transcription levels of these two gamma-CA genes. Low pH value (pH 7.5) significantly increased transcription level of Up gamma CA2 not Up gamma CA1 at 12 h, whereas high pH value (pH 8.5) significantly inhibited the transcription of these two gamma-CA genes at 6 h. These findings enhanced our understanding on transcriptional regulation of gamma-CA genes in response to environmental factors in U. prolifera.
关键词	Ulva prolifera Carbonic anhydrase Environmental factor Green tide Transcriptional regulation
DOI	10.1007/s10709-020-00112-4
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876165]; Strategic Priority Research Program of Chinese Academy of Sciences[XDA23050302/XDA23050403/XDB42000000]; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023]; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY020706]; Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province of China[SDAIT-26-09]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000607995300001
出版者	SPRINGER
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169972
专题	海洋生态与环境科学重点实验室
通讯作者	Liu, Feng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Marine Ecol & Environm Sci Lab, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China 4.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, Yu,Liu, Feng,Liu, Manman,et al. Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera[J]. GENETICA,2021:10.
APA	Wang, Yu,Liu, Feng,Liu, Manman,Shi, Shitao,Bi, Yuping,&Chen, Nansheng.(2021).Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera.GENETICA,10.
MLA	Wang, Yu,et al."Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera".GENETICA (2021):10.

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