Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems

doi:10.1016/j.ecoenv.2020.111617

IOCAS-IR > 实验海洋生物学重点实验室

	Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems
	Yu, Jiachen 1,2,3,4; Wang, Yanfeng 1,2,3; Xiao, Yongshuang 1,2,3; Li, Xian 1,2,3; Zhou, Li 1,2,3,4; Wang, Yunong 1,2,3,4; Du, Tengfei1,2,3,4 ; Ma, Xiaona 1,2,3; Li, Jun 1,2,3
	2021-01-15
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
卷号	208 页码:10
通讯作者	Li, Jun([email protected])
摘要	Nitrate (NO3-), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3- levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems (RAS). Fish were exposed to 0 mg/L (control, CK), 50 mg/L (low nitrate, LN), 200 mg/L (medium nitrate, MN), and 400 mg/L (high nitrate, HN) NO3-N for 60 d in experimental RAS. Cumulative survival (CS) was significantly decreased with increasing NO3- levels in LN, MN, and HN. The lowest CS was 35% in the HN group. Growth parameters, including absolute growth rate, specific growth rate, and feed conversion rate, were significantly different in HN compared with that in the CK. Histological survey of gills and liver revealed dose dependent histopathological damage induced by NO3- exposure and significant differences in glutamate pyruvate transaminase and glutamate oxalate transaminase in MN and HN compared with that in the CK. The hepatosomatic index in HN was significantly higher than that in the CK. Additionally, NO3- significantly increased bioaccumulation in plasma in LN, MN, and HN compared to that in the CK. Significant decreases in hemoglobin and increases in methemoglobin levels indicated reduced oxygen-carrying capacity in HN. Additionally, qRTPCR and enzyme-linked immunosorbent assay (ELISA) were developed to investigate key biomarkers involved in the GH/IGF-1, HPT, and HPI axes. Compared with that in the CK, the abundance of GH, GHRb, and IGF-1 was significantly lower in HN, whereas GHRa did not differ between treatments. The plasma T3 level significantly decreased in LN, MN, and HN and T4 significantly decreased in HN. The CRH, ACTH, and plasma cortisol levels were significantly upregulated in HN compared with that in the CK. We conclude that elevated NO3- exposure leads to growth retardation, impaired health status, and endocrine disorders in turbot and the NO3- level for juvenile turbot culture should not exceed 50 mg/L NO3-N in RAS. Our findings indicate that endocrine dysfunction of the GH/IGF-1, HPT, and HPI axes might be responsible for growth inhibition induced by NO3- exposure.
关键词	Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity
DOI	10.1016/j.ecoenv.2020.111617
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program[2018YFD0901204]; China Agriculture Research System[CARS-47-G21]; STS Project[KFZD-SW-106]; STS Project[ZSSD019]; STS Project[2017T3017]; STS Project[KFJ-STS-QYZX-020]; Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0502-2]; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]; National Natural Science Foundation of China[31672672]; Agricultural Application Technology Innovation Project of Shandong Province in 2018/2019[SD2019YY011]
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:000604135600010
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169890
专题	实验海洋生物学重点实验室
通讯作者	Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yu, Jiachen,Wang, Yanfeng,Xiao, Yongshuang,et al. Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,208:10.
APA	Yu, Jiachen.,Wang, Yanfeng.,Xiao, Yongshuang.,Li, Xian.,Zhou, Li.,...&Li, Jun.(2021).Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,208,10.
MLA	Yu, Jiachen,et al."Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 208(2021):10.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S014765132031（4720KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览