Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01

doi:10.1007/s00343-020-0203-6

IOCAS-IR > 实验海洋生物学重点实验室

	Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01
	Yue, Yang1 ; Yu, Huahua1,2 ; Li, Rongfeng 1,2; Hu, Linfeng 3; Liu, Song 1,2; Xing, Rong'e 1,2; Li, Pengcheng1,2
	2021-02-22
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	10
通讯作者	Yu, Huahua([email protected]) ; Li, Pengcheng([email protected])
摘要	Marine symbiotic fungi represent an intriguing source of discovery of novel secondary metabolites with various biological activities. Sea anemones are benthic marine invertebrates, however, the cultivable symbiotic fungi residing in the sea anemones are paid few attentions compared to those derived from their cnidarian counterparts. Here we show the identification of antimicrobial secondary metabolites from the sea anemone-derived symbiotic fungi. Out of five isolated fungal strains, only the strain SMA01 showed strong antimicrobial activities, which was assigned into the genus Emericella based on the morphological characteristics and the ITS sequencing. Media swift from liquid fermentation to solid rice medium presented little influence on its antibacterial activity. A chemical investigation of the ethyl acetate extract of the Emericella sp. SMA01 led to discovery of the primary antibiotic metabolite phenazine-1-carboxylic acid. The IC50 values of the phenazine-1-carboxylic acid against Phytophthora capsici, Gibberella zeae, and Verticillium dahliae were determined to be 23.26-53.89 mu g/mL. To the best of our knowledge, this was the first report of Emericella sp. in sea anemones. The current study may benefit understanding of the defensive chemical interactions between the symbiotic fungi and their host sea anemones.
关键词	sea anemone symbiotic fungi phenazine-1-carboxylic acid antimicrobial activity
DOI	10.1007/s00343-020-0203-6
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41376004]; National Natural Science Foundation of China[41406152]; Key Research and Development Project of Shandong Province[2018GHY115008]; Youth Innovation Promotion Association of CAS; Scientific and Technological Innovation Project[2015ASKJ02]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000620409100003
出版者	SCIENCE PRESS
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169725
专题	实验海洋生物学重点实验室
通讯作者	Yu, Huahua; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 3.Henan Inst Sci & Technol, Sch Chem & Chem Engn, Xinxiang 453003, Henan, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yue, Yang,Yu, Huahua,Li, Rongfeng,et al. Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2021:10.
APA	Yue, Yang.,Yu, Huahua.,Li, Rongfeng.,Hu, Linfeng.,Liu, Song.,...&Li, Pengcheng.(2021).Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,10.
MLA	Yue, Yang,et al."Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2021):10.

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