Transcriptomic analysis reveals the immune changes associated with reproduction in the clam Meretrix petechialis

doi:10.1016/j.fsi.2020.11.026

IOCAS-IR > 实验海洋生物学重点实验室

	Transcriptomic analysis reveals the immune changes associated with reproduction in the clam Meretrix petechialis
	Wang, Di 1,3; Liu, Baozhong1,2,3
	2021
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	108 页码:24-31
通讯作者	Liu, Baozhong([email protected])
摘要	Substantial mortality and economic losses in marine mollusk culture has drawn considerable attention in recent years. The changes in immune status and environmental stress are thought to be the main causes of shellfish summer mortality. The reproduction and immune defense are both physiologically demanding processes, therefore, the immune status of mollusk is likely to be affected by reproduction during breeding. In present study, we performed transcriptome and gene expression analyses in the clam Meretrix petechialis pre-/post-spawning. DEGs enrichment analysis revealed important immune signaling pathways and key genes changed after spawning. Further analysis showed females up-regulated genes involved in apoptosis, TLR signal pathway and heat shock, whereas males down-regulated complement-related genes after spawning. Additionally, both genders of clams up-regulated its immune response level to against Vibrio infection after spawning revealed by the changes of four immune-related DEGs. The up-regulation of two marker genes at the transcription and protein levels further confirmed that pathogen reinforced the expression differences of immune-related genes between the two groups. Our study provides a new insight into the understanding of molecular mechanisms underlying reproduction influenced immune differences in M. petechialis.
关键词	Transcriptome Reproduction Immunity Meretrix petechialis
DOI	10.1016/j.fsi.2020.11.026
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao); China Agriculture Research System[CARS-49]; National Natural Science Foundation of China[31772845]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000603053000004
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169552
专题	实验海洋生物学重点实验室
通讯作者	Liu, Baozhong
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Di,Liu, Baozhong. Transcriptomic analysis reveals the immune changes associated with reproduction in the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2021,108:24-31.
APA	Wang, Di,&Liu, Baozhong.(2021).Transcriptomic analysis reveals the immune changes associated with reproduction in the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,108,24-31.
MLA	Wang, Di,et al."Transcriptomic analysis reveals the immune changes associated with reproduction in the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 108(2021):24-31.

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