Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization

doi:10.1007/s13131-020-1683-0

	Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization
	Zhan, Zifeng 1,2,3; Xu, Kuidong1,2,3,4
	2020-12-28
发表期刊	ACTA OCEANOLOGICA SINICA
ISSN	0253-505X
页码	5
通讯作者	Xu, Kuidong([email protected])
摘要	The scuticociliatid ciliates Ancistrum haliotis and A. crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp. and the bivalve Ruditapes philippinarum. Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis. We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome, and optimized the fluorescence in situ hybridization (FISH) protocols for identification of A. halioti and A. crassum, respectively. The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes. The method can be used for quick and accurate quantitative analysis of A. haliotis and A. crassum infections on host molluscs.
关键词	oligonucleotide probes molecular methods scuticociliates SSU rRNA abalone mollusc
DOI	10.1007/s13131-020-1683-0
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876171]; National Natural Science Foundation of China[41406171]
WOS研究方向	Oceanography
WOS类目	Oceanography
WOS记录号	WOS:000603188400002
出版者	SPRINGER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169549
专题	海洋生物分类与系统演化实验室
通讯作者	Xu, Kuidong
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhan, Zifeng,Xu, Kuidong. Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization[J]. ACTA OCEANOLOGICA SINICA,2020:5.
APA	Zhan, Zifeng,&Xu, Kuidong.(2020).Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization.ACTA OCEANOLOGICA SINICA,5.
MLA	Zhan, Zifeng,et al."Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization".ACTA OCEANOLOGICA SINICA (2020):5.

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