Laboratory Culture-Based Characterization of the Resting Stage Cells of the Brown-Tide-Causing Pelagophyte, Aureococcus anophagefferens

doi:10.3390/jmse8121027

	Laboratory Culture-Based Characterization of the Resting Stage Cells of the Brown-Tide-Causing Pelagophyte, Aureococcus anophagefferens
	Ma, Zhaopeng 1,2,6; Hu, Zhangxi1,3,4 ; Deng, Yunyan 1,3,4; Shang, Lixia 1,3,4; Gobler, Christophere J.5; Tang, Ying Zhong 1,3,4
	2020-12-01
发表期刊	JOURNAL OF MARINE SCIENCE AND ENGINEERING
卷号	8 期号:12 页码:12
通讯作者	Hu, Zhangxi([email protected]) ; Tang, Ying Zhong([email protected])
摘要	Life history (life cycle) plays a vital role in the ecology of some microalgae; however, the well-known brown-tide-causing pelagophyte Aureococcus anophagefferens has been barely investigated in this regard. Recently, based mainly on detections in marine sediments from China, we proved that this organism has a resting stage. We, therefore, conducted a follow-up study to characterize the resting stage cells (RSCs) of A. anophagefferens using the culture CCMP1984. The RSCs were spherical, larger than the vegetative cells, and smooth in cell surface and contained more aggregated plastid but more vacuolar space than vegetative cells. RSCs contained a conspicuous lipid-enriched red droplet. We found a 9.9-fold decrease in adenosine triphosphate (ATP) content from vegetative cells to RSCs, indicative of a "resting" or dormant physiological state. The RSCs stored for 3 months (at 4 degrees C in darkness) readily reverted back to vegetative growth within 20 days after being transferred to the conditions for routine culture maintenance. Our results indicate that the RSCs of A. anophagefferens are a dormant state that differs from vegetative cells morphologically and physiologically, and that RSCs likely enable the species to survive unfavorable conditions, seed annual blooms, and facilitate its cosmopolitan distribution that we recently documented.
关键词	harmful algal blooms brown tide life history Aureococcus anophagefferens resting stage cell
DOI	10.3390/jmse8121027
收录类别	SCI
语种	英语
资助项目	Science & Technology Basic Resources Investigation Program of China[2018FY100200]; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504-2]; National Natural Science Foundation of China[61533011]; National Natural Science Foundation of China[41776125]; National Natural Science Foundation of China[41976134]
WOS研究方向	Oceanography
WOS类目	Oceanography
WOS记录号	WOS:000602096500001
出版者	MDPI
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169537
专题	海洋生态与环境科学重点实验室
通讯作者	Hu, Zhangxi; Tang, Ying Zhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.SUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11790 USA 6.Handan Univ, Sch Life Sci & Engn, Handan 056005, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Ma, Zhaopeng,Hu, Zhangxi,Deng, Yunyan,et al. Laboratory Culture-Based Characterization of the Resting Stage Cells of the Brown-Tide-Causing Pelagophyte, Aureococcus anophagefferens[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2020,8(12):12.
APA	Ma, Zhaopeng,Hu, Zhangxi,Deng, Yunyan,Shang, Lixia,Gobler, Christophere J.,&Tang, Ying Zhong.(2020).Laboratory Culture-Based Characterization of the Resting Stage Cells of the Brown-Tide-Causing Pelagophyte, Aureococcus anophagefferens.JOURNAL OF MARINE SCIENCE AND ENGINEERING,8(12),12.
MLA	Ma, Zhaopeng,et al."Laboratory Culture-Based Characterization of the Resting Stage Cells of the Brown-Tide-Causing Pelagophyte, Aureococcus anophagefferens".JOURNAL OF MARINE SCIENCE AND ENGINEERING 8.12(2020):12.

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