Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection | |
| Huang, Sijun1; Sun, Yingting1,2; Zhang, Si1; Long, Lijuan1 | |
| 2020-12-30 | |
| 发表期刊 | MICROBIOLOGYOPEN
 |
| ISSN | 2045-8827 |
| 页码 | 11 |
| 通讯作者 | Huang, Sijun([email protected]) ; Long, Lijuan([email protected]) |
| 摘要 | Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share similar gene expression patterns during infection. Whole-genome expression dynamics of T7-like cyanopodovirus P-SSP7 and its host Prochlorococcus strain MED4 have already been reported. Here, we conducted genomic and transcriptomic analyses on T7-like cyanopodovirus S-SBP1 during its infection on Synechococcus strain WH7803. S-SBP1 has a latent period of 8 h and phage DNA production of 30 copies per cell. In terms of whole-genome phylogenetic relationships and average nucleotide identity, S-SBP1 was most similar to cyanopodovirus S-RIP2, which also infects Synechococcus WH7803. Three hypervariable genomic islands were identified when comparing the genomes of S-SBP1 and S-RIP2. Single nucleotide variants were also observed in three S-SBP1 genes, which were located within the island regions. Based on RNA-seq analysis, S-SBP1 genes clustered into three temporal expression classes, whose gene content was similar to that of P-SSP7. Thirty-two host genes were upregulated during phage infection, including those involved in carbon metabolism, ribosome components, and stress response. These upregulated genes were similar to those upregulated by Prochlorococcus MED4 in response to infection by P-SSP7. Our study demonstrates a programmed temporal expression pattern of cyanopodoviruses and hosts during infection. |
| 关键词 | cyanophage genome cyanopodovirus RNA‐ seq Synechococcus transcriptome |
| DOI | 10.1002/mbo3.1150 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[41576126]; Natural Science Foundation of Guangdong Province[2017A030306020]; Youth Innovation Promotion Association[2018377]; Rising Star Foundation of the South China Sea Institute of Oceanology[NHXX2019ST0101]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA13020301] |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| WOS记录号 | WOS:000603422100001 |
| 出版者 | WILEY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/169502 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Huang, Sijun; Long, Lijuan |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Huang, Sijun,Sun, Yingting,Zhang, Si,et al. Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection[J]. MICROBIOLOGYOPEN,2020:11. |
| APA | Huang, Sijun,Sun, Yingting,Zhang, Si,&Long, Lijuan.(2020).Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection.MICROBIOLOGYOPEN,11. |
| MLA | Huang, Sijun,et al."Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection".MICROBIOLOGYOPEN (2020):11. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| mbo3.1150.pdf（745KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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