A live attenuated Edwardsiella tarda vaccine induces immunological expression pattern in Japanese flounder (Paralichthys olivaceus) in the early phase of immunization

doi:10.1016/j.cbpc.2020.108872

IOCAS-IR > 实验海洋生物学重点实验室

	A live attenuated Edwardsiella tarda vaccine induces immunological expression pattern in Japanese flounder (Paralichthys olivaceus) in the early phase of immunization
	Li, Xue-Peng; Zhang, Jian
	2021
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN	1532-0456
卷号	239 页码:12
通讯作者	Zhang, Jian([email protected])
摘要	A previous study showed that an attenuated Edwardsiella tarda strain, TXhfq, as a live vaccine could elicit protective immune effects in fish against E. tarda infection. In the current study, in order to clarify the molecular mechanism of fish immune response at the early stage after TXhfq vaccination, RNA-Seq technology was used to compare the transcriptomes of skin, intestine, and spleen between bath-vaccinated and unvaccinated Japanese flounder (Paralichthys olivaceus). An average of 46.6 million clean reads per library was obtained, similar to 88.04% of which were successfully mapped to the reference genome, and approximately 24,600 genes were detected in each sample. A total of 565, 878, and 1258 differential expression genes (DEGs) were found in skin, intestine, and spleen, respectively, including 1263 up-regulated genes and 1438 down-regulated genes. The DEGs exhibited different characteristics in each tissue. One hundred and sixteen DEGs belonging to six immune related categories were scrutinized, i.e., inflammatory factors, cytokines, complement and coagulation system, mucins, phagocytosis, and antigen processing and presentation. A protein-protein interaction network was constructed to get the interaction network between immune genes during the early stage of immunization. The top six hub genes highly regulated by TXhfq formed complicated interaction relationship with each other, which were involved in immune processes, notably inflammation and phagocytosis. Our results provide valuable information for the understanding of the immune mechanism underlying the protection of live attenuated vaccines in fish.
关键词	Paralichthys olivaceus Transcriptome Attenuated vaccine Immune response
DOI	10.1016/j.cbpc.2020.108872
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-3]; National Marine Economy Innovation and Development Regional Demonstration Project (Qingdao); Taishan Scholar Program of Shandong Province
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS记录号	WOS:000588051700012
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169223
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Jian
作者单位	1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Yantai Univ, Sch Ocean, Yantai, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Xue-Peng,Zhang, Jian. A live attenuated Edwardsiella tarda vaccine induces immunological expression pattern in Japanese flounder (Paralichthys olivaceus) in the early phase of immunization[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2021,239:12.
APA	Li, Xue-Peng,&Zhang, Jian.(2021).A live attenuated Edwardsiella tarda vaccine induces immunological expression pattern in Japanese flounder (Paralichthys olivaceus) in the early phase of immunization.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,239,12.
MLA	Li, Xue-Peng,et al."A live attenuated Edwardsiella tarda vaccine induces immunological expression pattern in Japanese flounder (Paralichthys olivaceus) in the early phase of immunization".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 239(2021):12.

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