Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder-Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection

doi:10.3390/ijms21207725

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	Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder-Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection
	Li, Wenrui1,2,3 ; Guan, Xiaolu 1,2; Sun, Li 1,2,3
	2020-10-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	21 期号:20 页码:18
通讯作者	Sun, Li([email protected])
摘要	MicroRNAs (miRNAs) are small non-coding RNAs with important roles in diverse biological processes including immunity. Japanese flounder (Paralichthys olivaceus) is an aquaculture fish species susceptible to the infection of bacterial and viral pathogens including Edwardsiella tarda. In a previous study, pol-miR-novel_547, a novel miRNA of flounder with unknown function, was found to be induced by E. tarda. In the present study, we investigated the regulation and function of pol-miR-novel_547 and its target gene. We found that pol-miR-novel_547 was regulated differently by E. tarda and the viral pathogen megalocytivirus, and pol-miR-novel_547 repressed the expression of PTEN (phosphatase and tensin homolog) of flounder (PoPTEN). PoPTEN is ubiquitously expressed in multiple tissues of flounder and responded to bacterial and viral infections. Interference with PoPTEN expression in flounder cells directly or via pol-miR-novel_547 promoted E. tarda invasion. Consistently, in vivo knockdown of PoPTEN enhanced E. tarda dissemination in flounder tissues, whereas in vivo overexpression of PoPTEN attenuated E. tarda dissemination but facilitated megalocytivirus replication. Further in vitro and in vivo studies showed that PoPTEN affected autophagy activation via the AKT/mTOR pathway and also modulated the process of apoptosis. Together these results reveal for the first time a critical role of fish PTEN and its regulatory miRNA in pathogen infection, autophagy, and apoptosis.
关键词	Paralichthys olivaceus microRNA phosphatase and tensin homolog pathogen infection immune defense autophagy apoptosis
DOI	10.3390/ijms21207725
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31730100]; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2]; Taishan Scholar Program of Shandong Province
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000585514600001
出版者	MDPI
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169130
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, CAS Ctr Ocean Megasci, CAS Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Wenrui,Guan, Xiaolu,Sun, Li. Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder-Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(20):18.
APA	Li, Wenrui,Guan, Xiaolu,&Sun, Li.(2020).Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder-Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(20),18.
MLA	Li, Wenrui,et al."Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder-Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.20(2020):18.

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