Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells

doi:10.1016/j.carbpol.2020.117098

IOCAS-IR > 实验海洋生物学重点实验室

	Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells
	Xu, Chaojie1,2,3 ; Xing, Ronge1,2 ; Liu, Song 1,2; Qin, Yukun1,2 ; Li, Kecheng 1,2; Yu, Huahua1,2 ; Li, Pengcheng1,2
	2021
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	251 页码:11
通讯作者	Xing, Ronge([email protected]) ; Li, Pengcheng([email protected])
摘要	In this study, we synthesized negatively charged chitosan sulfate and positively charged hydroxypropyltrimethyl ammonium chloride chitosan (HACC), and then prepared chitosan derivatives with positive and negative ions as nanoparticles (NPs) by ovalbumin encapsulation using the polyelectrolyte method. NPs with different substitution sites and molecular weights (MW) were prepared by varying conditions. We then determined the zeta potential average, diameter, encapsulation effect, and their immunostimulatory effects on dendritic cells (DCs). The results showed that chitosan-derivative NPs ranged in size from 153.33 to 320.90 nm; all NPs were positive, with charges ranging from 17.10 to 39.30 mV and the encapsulation rates of 65 %-75 %. Three NPs greatly promoted the expression and secretion of interleukin-6 (IL-6), tumor necrosis factor (TNF-alpha), and interleukin-1 beta (IL-1 beta) in DC cells: C2,3,6 chitosan sulfate-HACC (C2,3,6-HACC; 200 kDa), C3,6 chitosan sulfate-HACC (C3,6-HACC; 200 kDa) and C6 chitosan sulfate-HACC (C6-HACC; 50 kDa). We also found that 200-kDa C2,3,6-HACC and 50-kDa C6-HACC NPs greatly increased secretion of the major histocompatibility complex-II (MHC-II), CD40, CD80, and CD86, indicating that these NPs promote effective antigen presentation, further increasing immunity effects. Finally, we applied laser confocal photography and determined that NPs entered the cell to promote the regulation of cellular immune activity; this discovery lays a foundation for further research on their mechanism of their action. Therefore, C2,3,6-HACC and C6-HACC NPs have the potential as immunological adjuvants.
关键词	Chitosan quaternary ammonium salt Sulfated chitosan Nanoparticles DCS cells Immunostimulatory activities
DOI	10.1016/j.carbpol.2020.117098
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFC0311305]; Key Research and Development Program of Shandong Province[2019GHY112015]; Key Research and Development Program of Shandong Province[2019YYSP028]
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000582801500092
出版者	ELSEVIER SCI LTD
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169067
专题	实验海洋生物学重点实验室
通讯作者	Xing, Ronge; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Xu, Chaojie,Xing, Ronge,Liu, Song,et al. Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells[J]. CARBOHYDRATE POLYMERS,2021,251:11.
APA	Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2021).Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells.CARBOHYDRATE POLYMERS,251,11.
MLA	Xu, Chaojie,et al."Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells".CARBOHYDRATE POLYMERS 251(2021):11.

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