| Endosymbionts of Metazoans Dwelling in the PACManus Hydrothermal Vent: Diversity and Potential Adaptive Features Revealed by Genome Analysis | |
Li, Leilei1; Wang, Minxiao1,2,3; Li, Lifeng4; Du, Zengfeng5,6,7; Sun, Yan1,2,3; Wang, Xiaocheng8 ; Zhang, Xin2,5,6,7,9; Li, Chaolun1,2,3,6,9
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| 2020-11-01 | |
| 发表期刊 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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| ISSN | 0099-2240 |
| 卷号 | 86期号:21页码:22 |
| 通讯作者 | Zhang, Xin([email protected]) ; Li, Chaolun([email protected]) |
| 摘要 | Deep-sea hydrothermal vent communities are dominated by invertebrates, namely, bathymodiolin mussels, siboglinid tubeworms, and provannid snails. Symbiosis is considered key to successful colonization by these sedentary species in such extreme environments. In the PACManus vent fields, snails, tubeworms, and mussels each colonized a niche with distinct geochemical characteristics. To better understand the metabolic potentials and genomic features contributing to host-environment adaptation, we compared the genomes of the symbionts of Bathymodiolus manusensis, Arcovestia ivanovi, and Alviniconcha boucheti sampled at PACManus, and we discuss their environmentally adaptive features. We found that B. manusensis and A. ivanovi are colonized by Gammaproteobacteria from distinct clades, whereas endosymbionts of B. manusensis feature high intraspecific heterogeneity with differing metabolic potentials. A. boucheti harbored three novel Epsilonproteobacteria symbionts, suggesting potential species-level diversity of snail symbionts. Genome comparisons revealed that the relative abundance of gene families related to low-pH homeostasis, metal resistance, oxidative stress resistance, environmental sensing/responses, and chemotaxis and motility was the highest in A. ivanovi's symbiont, followed by symbionts of the vent-mouth-dwelling snail A. boucheti, and was relatively low in the symbiont of the vent-periphery-dwelling mussel B. manusensis, which is consistent with their environmental adaptations and host-symbiont interactions. Gene families classified as encoding host interaction/attachment, virulence factors/toxins, and eukaryotic-like proteins were most abundant in symbionts of mussels and least abundant in those of snails, indicating that these symbionts may differ in their host colonization strategies. Comparison of Epsilonproteobacteria symbionts to nonsymbionts demonstrated that the expanded gene families in symbionts were related to vitamin B12 synthesis, toxin-antitoxin systems, methylation, and lipopolysaccharide biosynthesis, suggesting that these are vital to symbiont establishment and development in Epsilonproteobacteria. IMPORTANCE Deep-sea hydrothermal vents are dominated by several invertebrate species. The establishment of symbiosis has long been thought to be the key to successful colonization by these sedentary species in such harsh environments. However, the relationships between symbiotic bacteria and their hosts and their role in environmental adaptations generally remain unclear. In this paper, we show that the distribution of three host species showed characteristic niche partitioning in the Manus Basin, giving us the opportunity to understand how they adapt to their particular habitats. This study also revealed three novel genomes of symbionts from the snails of A. boucheti. Combined with a data set on other ectosymbiont and free-living bacteria, genome comparisons for the snail endosymbionts pointed to several genetic traits that may have contributed to the lifestyle shift of Epsilonproteobacteria into the epithelial cells. These findings could increase our understanding of invertebrate-endosymbiont relationships in deep-sea ecosystems. |
| 关键词 | endosymbionts genome analysis hydrothermal vents environmental geochemical gradient |
| DOI | 10.1128/AEM.00815-20 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303]; National Key R&D Program of the Ministry of Science and Technology[2018YFC0310802]; Open Research Project of the National Major Science & Technology Infrastructure[NMSTI-KEXUE2017K01]; Senior User Project of RV KEXUE[KEXUE201XGXX]; National Natural Science Foundation of China[41806200]; National Natural Science Foundation of China[41822604]; CAS Interdisciplinary Innovation Team[JCTD-2018-12]; State Key Laboratory of Microbial Technology Open Projects Fund[M2019-07] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000582928500002 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/168981 |
| 专题 | 海洋地质与环境重点实验室 |
| 通讯作者 | Zhang, Xin; Li, Chaolun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 4.Beijing GensKey Technol Co Ltd, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao, Peoples R China 6.Chinese Acad Sci, Deep Sea Res Ctr, Inst Oceanol, Qingdao, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao, Peoples R China 8.Natl Marine Environm Monitoring Ctr, Dalian, Peoples R China 9.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心; 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Leilei,Wang, Minxiao,Li, Lifeng,et al. Endosymbionts of Metazoans Dwelling in the PACManus Hydrothermal Vent: Diversity and Potential Adaptive Features Revealed by Genome Analysis[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2020,86(21):22. |
| APA | Li, Leilei.,Wang, Minxiao.,Li, Lifeng.,Du, Zengfeng.,Sun, Yan.,...&Li, Chaolun.(2020).Endosymbionts of Metazoans Dwelling in the PACManus Hydrothermal Vent: Diversity and Potential Adaptive Features Revealed by Genome Analysis.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,86(21),22. |
| MLA | Li, Leilei,et al."Endosymbionts of Metazoans Dwelling in the PACManus Hydrothermal Vent: Diversity and Potential Adaptive Features Revealed by Genome Analysis".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 86.21(2020):22. |
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| Applied and Environm（9643KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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