A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)

doi:10.1111/jpy.13066

IOCAS-IR > 实验海洋生物学重点实验室

	A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)
	Shan, Tifeng ; Pang, Shaojun 1; Wang, Xuemei ; Li, Jing; Li, Qianxi; Su, Li ; Li, Xiaodong
	2020-10-27
发表期刊	JOURNAL OF PHYCOLOGY
ISSN	0022-3646
页码	6
通讯作者	Pang, Shaojun([email protected])
摘要	Studies of quantitative trait loci based on genetic linkage maps require the establishment of a mapping population. Permanent mapping populations are more ideal than temporary ones because they can be used repeatedly. However, there has been no reported permanent sporophyte population of economically important kelp species. Based on the characteristics of the kelp life cycle, we proposed a method to establish, and then constructed experimentally, an "immortalized F-2" (IF2) population of Undaria pinnatifida. Doubled-haploid "female" and "male" sporophytes were obtained through the parthenogenesis of a female gametophyte clone and the selfing of a "monoicous" gametophyte clone (originally male), respectively, and they were used as the parents. The F-1 hybrid line was generated by crossing the female and male gametophytes derived from the respective female and male parents. Full-sibling F-2 gametophyte clones, consisting of 260 females and 260 males, were established from an F-1 hybrid sporophyte. Thirty-five females and 35 males were randomly selected and paired to give rise to an IF2 population containing 35 crossing lines. A parentage analysis using 10 microsatellite markers confirmed the accuracy of the IF2 population and indicated the feasibility of this method. This proposed method may be adapted for use in other kelp species, and thus, it will be useful for genetic studies of kelp.
关键词	breeding kelp macroalgae permanent population quantitative trait loci seaweed
DOI	10.1111/jpy.13066
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41676128]; Biological Resources Program from Chinese Academy of Sciences[KFJ-BRP-017-27]; China Agriculture Research System[CARS-50]; Taishan Scholar Program of Shandong Province; Foundation for Huiquan Scholar of Institute of Oceanology, Chinese Academy of Sciences
WOS研究方向	Plant Sciences ; Marine & Freshwater Biology
WOS类目	Plant Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000583511200001
出版者	WILEY
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168978
专题	实验海洋生物学重点实验室
通讯作者	Pang, Shaojun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Shan, Tifeng,Pang, Shaojun,Wang, Xuemei,et al. A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)[J]. JOURNAL OF PHYCOLOGY,2020:6.
APA	Shan, Tifeng.,Pang, Shaojun.,Wang, Xuemei.,Li, Jing.,Li, Qianxi.,...&Li, Xiaodong.(2020).A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae).JOURNAL OF PHYCOLOGY,6.
MLA	Shan, Tifeng,et al."A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)".JOURNAL OF PHYCOLOGY (2020):6.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
jpy.13066.pdf（417KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览