A novel microRNA and its pfk target control growth length in the freshwater shrimp Neocaridina heteropoda

doi:10.1242/jeb.223529

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	A novel microRNA and its pfk target control growth length in the freshwater shrimp Neocaridina heteropoda
	Li, Ran 1; Weng, Jieyang 2; Ren, Liqi 3; Wang, Xin 1; Meng, Qinghao 1; Wang, Liyan 1; Sun, Jinsheng 1
	2020-07-01
发表期刊	JOURNAL OF EXPERIMENTAL BIOLOGY
ISSN	0022-0949
卷号	223 期号:13 页码:8
通讯作者	Sun, Jinsheng([email protected])
摘要	MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression and play roles in a wide range of physiological processes, including ontogenesis. Herein, we discovered a novel miRNA, novel miR-26, which inhibits translation of the phosphofructokinase (PFK) gene by targeting the 3' untranslated region (UTR) of pfk directly, thereby inhibiting molting and body length growth of the freshwater shrimp Neocaridina heteropoda. Lowering expression of pfk by RNA interference (RNAi) led to a longer ecdysis cycle and smaller individuals. This phenotype was mirrored in shrimps injected with novel miR-26 agomirs, but the opposite phenotype occurred in shrimps injected with novel miR-26 antagomirs (i.e. the ecdysis cycle was shortened and body length was increased). After injection of 20-hydroxyecdysone (ecdysone 20E), expression of the novel miR-26 was decreased, while expression of pfk was up-regulated, and the fructose-1,6-diphosphate metabolite of PFK accumulated correspondingly. Furthermore, expression of eIF2 (eukaryotic initiation factor 2) increased under stimulation with fructose-1,6-diphosphate, suggesting that protein synthesis was stimulated during this period. Taken together, our results suggest that the novel miR-26 regulates expression of pfk and thereby mediates the molting and growth of N. heteropoda.
关键词	Phosphofructokinase RNAi Ecdysis Molting Crustacean Body length EIF2 EIF2B
DOI	10.1242/jeb.223529
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901301]; Innovation Team of Tianjin Fisheries Research System[ITTFRS2017007]; Tianjin Development Program for Innovation and Entrepreneurship[TD13-5076]; Tianjin Natural Science Foundation[17JCYBJC29700]
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:000576693100020
出版者	COMPANY BIOLOGISTS LTD
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168936
专题	中国科学院海洋研究所
通讯作者	Sun, Jinsheng
作者单位	1.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Beijing 101 Middle Sch, Beijing 100091, Peoples R China
推荐引用方式 GB/T 7714	Li, Ran,Weng, Jieyang,Ren, Liqi,et al. A novel microRNA and its pfk target control growth length in the freshwater shrimp Neocaridina heteropoda[J]. JOURNAL OF EXPERIMENTAL BIOLOGY,2020,223(13):8.
APA	Li, Ran.,Weng, Jieyang.,Ren, Liqi.,Wang, Xin.,Meng, Qinghao.,...&Sun, Jinsheng.(2020).A novel microRNA and its pfk target control growth length in the freshwater shrimp Neocaridina heteropoda.JOURNAL OF EXPERIMENTAL BIOLOGY,223(13),8.
MLA	Li, Ran,et al."A novel microRNA and its pfk target control growth length in the freshwater shrimp Neocaridina heteropoda".JOURNAL OF EXPERIMENTAL BIOLOGY 223.13(2020):8.

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