Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition

doi:10.1111/1462-2920.15237

IOCAS-IR > 实验海洋生物学重点实验室

	Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition
	Wu, Zuodong 1; Zheng, Rikuan2,3,4 ; Liu, Ge 2,3,4; Liu, Rui 2,3,4; Wu, Shimei 1; Sun, Chaomin 2,3,4
	2020-09-29
发表期刊	ENVIRONMENTAL MICROBIOLOGY
ISSN	1462-2912
页码	13
通讯作者	Wu, Shimei([email protected]) ; Sun, Chaomin([email protected])
摘要	Cadmium (Cd) is a common toxic heavy metal in the environment, and bacteria have evolved different strategies against Cd-toxicity. Here, we found that marine bacteriumBacillussp. 98 could significantly alleviate Cd-toxicity by recruiting calcium (Ca) for reducing excessive intracellular nitric oxide (NO) and enhancing iron acquisition. To investigate the underlying mechanisms, mass spectrometry-based proteomic analysis was applied toBacillussp. 98 after treated with Cd supplemented with or without Ca. Compared with bacterial cells treated with Cd only, the proteomic results showed that the expression level of NO synthase was markedly down-regulated, while the expression levels of NO dioxygenase, which is responsible for converting NO to nitrate, and proteins associated with iron uptake were profoundly enhanced when Ca was supplemented. Consistently, bacterial intracellular NO amount was dramatically increased afterBacillussp. 98 was treated with Cd, and reversed to a normal level when Ca or iron was supplemented. Notably, Ca also protected bacteria against stresses from other heavy metals including Cu, Cr, Mn, Ni and Zn, and this self-protection strategy was adopted as well in zebrafish, which encourages us to develop Ca-associated products against heavy metals toxicity in the future.
DOI	10.1111/1462-2920.15237
收录类别	SCI
语种	英语
资助项目	China Ocean Mineral Resources R&D Association Grant[DY135-B2-14]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301]; National Key R and D Program of China[2018YFC0310800]; Taishan Young Scholar Program of Shandong Province[tsqn20161051]; Qingdao Innovation Leadership Program[18-1-2-7-zhc]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000573469300001
出版者	WILEY
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168859
专题	实验海洋生物学重点实验室
通讯作者	Wu, Shimei; Sun, Chaomin
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wu, Zuodong,Zheng, Rikuan,Liu, Ge,et al. Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition[J]. ENVIRONMENTAL MICROBIOLOGY,2020:13.
APA	Wu, Zuodong,Zheng, Rikuan,Liu, Ge,Liu, Rui,Wu, Shimei,&Sun, Chaomin.(2020).Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition.ENVIRONMENTAL MICROBIOLOGY,13.
MLA	Wu, Zuodong,et al."Calcium protects bacteria against cadmium stress via reducing nitric oxide production and increasing iron acquisition".ENVIRONMENTAL MICROBIOLOGY (2020):13.

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