Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar

doi:10.1016/j.theriogenology.2020.06.007

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	Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar
	Wu, Zhihao 1,2; Xu, Shihong 1,2; Wang, Lijuan1,2 ; Wu, Qiaowan1,2,3 ; Li, Jun 1,2; Liu, Hao 4; You, Feng 1,2
	2020-10-01
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	155 页码:49-59
通讯作者	You, Feng([email protected])
摘要	Triploid Atlantic salmon Salmo salar is commercially available and ideal means of achieving sterility in fish. The expression patterns of triploid parr and smolt were described before. However, little is known about the gene expression and fatty acid composition of triploid alevins at endogenous nutritional stage, which is an important period for their survival and development. In this study, the development of the diploid and triploid Atlantic salmon embryos and hatched alevins before feeding was compared, and the results showed that there was no obvious morphological difference between them. And then, the transcription profiles of the triploid and diploid alevins on 3, 23 and 33 days post hatching (dph) were investigated by using RNA-seq after the yolk sacs were removed. There were more differentially expressed genes (DEGs) on 3 dph (2925) than those on 23 (589) and 33 dph (606). Compared with the diploid groups, the up-regulated genes in the triploid groups at the three sampling times were 1189, 339 and 340, respectively, while 1736, 250 and 266 genes were down-regulated. There were 2088 and 5215 DEGs on 23 and 33 dph compared with on 3 dph in the diploid groups, while 6533 and 9340 DEGS on 23 and 33 dph compared with 3 dph in the triploid groups. The functional enrichment analysis showed that the DEGs were mainly enriched to digestion and metabolism function, especially on 33 dph when the larvae were about to feeding. Meanwhile, 15 out of 22 kinds of fatty acids of alevins on 33 dph showed significant differences (P < 0.05), in which all the fatty acids contents in the triploid were lower. The results indicated the specific nutritional requirement in the triploid was represented as early as yolk sac stage. The present study gave an important vision in triploid Atlantic salmon breeding. (C) 2020 Elsevier Inc. All rights reserved.
关键词	Atlantic salmon Salmo salar Triploid Gene expression Fatty acid composition
DOI	10.1016/j.theriogenology.2020.06.007
收录类别	SCI
语种	英语
资助项目	Key research and development plan of Shandong province[2017CXGC0101]; National Natural Science Foundation of China[31772834]; National Key R&D Program of China[2018YFD0901202]
WOS研究方向	Reproductive Biology ; Veterinary Sciences
WOS类目	Reproductive Biology ; Veterinary Sciences
WOS记录号	WOS:000566760000007
出版者	ELSEVIER SCIENCE INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168769
专题	实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wu, Zhihao,Xu, Shihong,Wang, Lijuan,et al. Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar[J]. THERIOGENOLOGY,2020,155:49-59.
APA	Wu, Zhihao.,Xu, Shihong.,Wang, Lijuan.,Wu, Qiaowan.,Li, Jun.,...&You, Feng.(2020).Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar.THERIOGENOLOGY,155,49-59.
MLA	Wu, Zhihao,et al."Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar".THERIOGENOLOGY 155(2020):49-59.

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