Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis

doi:10.1016/j.jprot.2020.103891

IOCAS-IR > 实验海洋生物学重点实验室

	Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis
	Bao, Chenchang 1; Song, Chengwen2 ; Liu, Yuan 2; Yang, Yanan 1; Cui, Zhaoxia 1,3
	2020-08-30
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
卷号	226 页码:9
通讯作者	Cui, Zhaoxia([email protected])
摘要	Lysine crotonylation (Kcr) is a recently-discovered type of post-translational modification. Although Kcr has been reported in many species, little is known about this process in crustaceans. In this study, pan anti-lysine crotonylation antibody enrichment and high-resolution liquid chromatogram-mass spectrometry analysis were employed to characterize Kcr in testis of the Chinese mitten crab Eriocheir sinensis testis. Overall, 2799 Kcr sites were identified on 908 proteins with 14 conserved motifs. Bioinformatics analysis showed that Kcr was predominant on proteins found in cytoplasm, mitochondria and nucleus, and those involved in ribosome, proteasome, carbon metabolism and protein processing in endoplasmic reticulum. In total, 83 up-regulated and 12 down-regulated non-histone crotonylated sites were identified during spermiogenesis. These differentially expressed proteins were enriched in protein processing in endoplasmic reticulum pathway during formation of acrosome. In contrast, histone Kcr associated with mammalian spermatogenesis. These results provide foundational knowledge on the role of non-histone Kcr in spermiogenesis of E. sinensis. Significance: Lysine crotonylation (Kcr) is a recently-identified post-translational modification, and histone Kcr was found to associate with mammalian spermatogenesis. However, crotonylation of non-histone proteins has not been reported in spermatogenesis regulation. Further, there is no information on crotonylation in crustaceans. This study was the first large-scale Kcr proteome characterization in crustaceans. A total of 2799 Kcr sites on 908 proteins with 14 conserved motifs were identified from Eriocheir sinensis testis. Of which, 83 up-regulated and 12 down-regulated non-histone crotonylated sites were identified during spermiogenesis. Our results provide the basic information for further functional validation of Kcr proteins and revealed new roles of Kcr in spermiogenesis of E. sinensis.
关键词	Lysine crotonylation Proteomics Spermiogenesis Eriocheir sinensis
DOI	10.1016/j.jprot.2020.103891
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0900303]; Ten Thousand Talents Program; K. C. Wong Magna Fund in Ningbo University
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000566934000008
出版者	ELSEVIER
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168750
专题	实验海洋生物学重点实验室
通讯作者	Cui, Zhaoxia
作者单位	1.Ningbo Univ, Sch Marine Sci, Ningbo 315020, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Bao, Chenchang,Song, Chengwen,Liu, Yuan,et al. Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis[J]. JOURNAL OF PROTEOMICS,2020,226:9.
APA	Bao, Chenchang,Song, Chengwen,Liu, Yuan,Yang, Yanan,&Cui, Zhaoxia.(2020).Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis.JOURNAL OF PROTEOMICS,226,9.
MLA	Bao, Chenchang,et al."Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis".JOURNAL OF PROTEOMICS 226(2020):9.

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