Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation

doi:10.2216/09-91.1

IOCAS-IR > 实验海洋生物学重点实验室

	Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation
	Wang, Jinfeng1,2 ; Jiang, Peng 1; Cui, Yulin 1,2; Guan, Xiangyu 1; Qin, Song 1; Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
	2010-07-01
发表期刊	PHYCOLOGIA
ISSN	0031-8884
卷号	49 期号:4 页码:355-360
文章类型	Article
摘要	Genetic transformation by electroporation of protoplasts is a standard procedure for many plants. However, for the genus Porphyra, the method is not effective because of low viability of protoplasts and is a time-consuming and expensive procedure. Based on the life history of Porphyra, a spore-targeted strategy of genetic transformation was developed, that is, using fresh conchospores of Porphyra haitanensis Chang & Zheng transformed by agitation with glass beads. A SV40 promoter-driven lacZ reporter gene was expressed in conchospores 48 h after the agitation. More transformants were obtained by increasing the agitation time from 10 to 25 s. The maximum number of transformants was more than six out of 1 million agitated conchospores. Transfer of a SV40 promoter-driven egfp gene into conchospores resulted in significant green GFP fluorescence. The expression of lacZ and egfp revealed that this strategy of spore-targeted transformation using glass bead agitation is feasible in P. haitanensis and that the SV40 promoter is effective for monitoring expression of foreign genes in this red algal species.; Genetic transformation by electroporation of protoplasts is a standard procedure for many plants. However, for the genus Porphyra, the method is not effective because of low viability of protoplasts and is a time-consuming and expensive procedure. Based on the life history of Porphyra, a spore-targeted strategy of genetic transformation was developed, that is, using fresh conchospores of Porphyra haitanensis Chang & Zheng transformed by agitation with glass beads. A SV40 promoter-driven lacZ reporter gene was expressed in conchospores 48 h after the agitation. More transformants were obtained by increasing the agitation time from 10 to 25 s. The maximum number of transformants was more than six out of 1 million agitated conchospores. Transfer of a SV40 promoter-driven egfp gene into conchospores resulted in significant green GFP fluorescence. The expression of lacZ and egfp revealed that this strategy of spore-targeted transformation using glass bead agitation is feasible in P. haitanensis and that the SV40 promoter is effective for monitoring expression of foreign genes in this red algal species.
关键词	Egfp Genetic Transformation Lacz Porphyra Haitanensis Sv40 Promoter
学科领域	Plant Sciences ; Marine & Freshwater Biology
DOI	10.2216/09-91.1
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000280232300006
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1683
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
通讯作者	Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jinfeng,Jiang, Peng,Cui, Yulin,et al. Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation[J]. PHYCOLOGIA,2010,49(4):355-360.
APA	Wang, Jinfeng,Jiang, Peng,Cui, Yulin,Guan, Xiangyu,Qin, Song,&Jiang, P, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.(2010).Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation.PHYCOLOGIA,49(4),355-360.
MLA	Wang, Jinfeng,et al."Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation".PHYCOLOGIA 49.4(2010):355-360.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Wang-2010-Gene trans（1708KB）			限制开放	--	浏览