Rotifers release a lipid-soluble agent that inhibits photosynthetic electron transport inChlorellasp

doi:10.1007/s10811-020-02065-9

IOCAS-IR > 实验海洋生物学重点实验室

	Rotifers release a lipid-soluble agent that inhibits photosynthetic electron transport inChlorellasp
	Xu, Ran 1,2; Zhang, Litao 1,3; Liu, Jianguo 1,3
	2020-03-10
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
页码	9
摘要	Rotifer contamination, as a severe constraint in mass cultures, impedes industrial-scale cultivation of microalgae. Once contamination occurred, the rotifers released something that inhibited microalgal cell growth in addition to algal reduction due to predation. With the aim of further identifying the type and effects of the inhibitor released from rotifers,Chlorellasp. was cultured in fresh modified F/2 medium and in media containing various proportions of boiled rotifer culture-medium filtrate (RCF), as well as in media containing water-soluble or lipid-soluble fractions isolated from RCF. Then, the growth and photosynthesis ofChlorellasp. were measured. The rotifer inhibition ofChlorellagrowth could not be eliminated by boiling the RCF, indicating that the inhibitor was not likely a protein. The growth and photosynthesis ofChlorellasp. was not inhibited by the water-soluble RCF fraction, but they were significantly inhibited by the lipid-soluble RCF fraction, in a dose-dependent manner. Further, the lipid-soluble fraction decreased energy conservation and photosynthetic electron transport, which induced a severe decrease in PSII activity and a decrease in the net photosynthetic O(2)evolution rate. Based on these physiological responses ofChlorellacells, the lipid-soluble fraction rather than the protein or water-soluble fractions was determined to contain the responsible inhibitor, suggesting the direction of further studies.
关键词	Chlorellasp Rotifer-derived growth inhibition Lipid-soluble inhibitor Photosynthetic inhibition mechanism
DOI	10.1007/s10811-020-02065-9
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[U1706209]; National Key Research and Development Program-China[2016YFB0601004]; Shandong Provincial Natural Science Foundation, China[ZR2019BC057]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000562590800002
出版者	SPRINGER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/168295
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Xu, Ran,Zhang, Litao,Liu, Jianguo. Rotifers release a lipid-soluble agent that inhibits photosynthetic electron transport inChlorellasp[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:9.
APA	Xu, Ran,Zhang, Litao,&Liu, Jianguo.(2020).Rotifers release a lipid-soluble agent that inhibits photosynthetic electron transport inChlorellasp.JOURNAL OF APPLIED PHYCOLOGY,9.
MLA	Xu, Ran,et al."Rotifers release a lipid-soluble agent that inhibits photosynthetic electron transport inChlorellasp".JOURNAL OF APPLIED PHYCOLOGY (2020):9.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
Xu2021_Article_Rotif（1119KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览