Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage | |
Zhang, Chunhui1 ; Li, Runzhi1; Zhu, Qin1; Hang, Wei1; Zhang, Hongjiang1; Cui, Hongli1; Ji, Chunli1; Zhang, Litao2; Chen, Fangjian3
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| 2020-09-01 | |
| 发表期刊 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| ISSN | 2211-9264 |
| 卷号 | 50页码:8 |
| 摘要 | During outdoor large-scale cultivation, Haematococcus pluvialis cells, especially at the green motile stage, are frequently exposed to chilling stress in winter. However, the physiological responses and adaptation mechanisms to chilling in H. pluvialis have not been characterized. In order to better understand the chilling tolerance mechanisms of H. pluvialis in the green motile stage, the responses of photosynthetic characteristics and photo-protective mechanisms to chilling were investigated. Chilling stress significantly decreased the activities of key enzymes (ribulose-1,5-bisphosphate carboxylase/oxygenase and NADP glyceraldehyde-3-phosphate dehydrogenase) in photosynthetic carbon assimilation, which would cause the accumulation of excess reducing equivalents and an imbalance of light absorption and energy utilization, leading to more severe photoinhibition. After chilling treatment, there was no induction of cyclic electron flow, non-photochemical quenching, fluorescence emission, or catalase, indicating these factors do not protect H. pluvialis from photoinhibition. However, chilling significantly enhanced the activities of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase), which efficiently dissipated excess electrons generated by photosynthetic linear electron flow (LEF). The malate-oxaloacetate shuttle was activated and the mitochondrial alternative oxidase (AOX) pathway was significantly up-regulated after chilling, indicating the transport of excess reducing equivalents generated by photosynthetic LEF to cytosol and mitochondria and oxidization by the AOX pathway, the mitochondrial non-phosphorylating pathway. As a result, the LEF was not inhibited by negative feedback and the H. pluvialis cells were well protected under chilling stress in the light, with no change in the effective quantum yield of PSII (FPSII) and the yield of non-regulated energy dissipation of PSII (FNO). The results indicate that antioxidant enzymes and the mitochondrial AOX pathway play important roles in protecting H. pluvialis at the green motile stage against chilling stress. |
| 关键词 | Chilling tolerance Microalgae Photoinhibition Photoprotection Reducing equivalents |
| DOI | 10.1016/j.algal.2020.102003 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key Research and Development Program of China[2018YFE0107200]; National Natural Science Foundation of China[31872588]; Shandong Provincial Natural Science Foundation, China[ZR2019BC057]; Doctoral Incentive Funding Research Project of Shanxi Province[SXYBKY2019037] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000558684000002 |
| 出版者 | ELSEVIER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/168184 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, Runzhi; Zhang, Litao |
| 作者单位 | 1.Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Taigu 030801, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Zhang, Chunhui,Li, Runzhi,Zhu, Qin,et al. Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2020,50:8. |
| APA | Zhang, Chunhui.,Li, Runzhi.,Zhu, Qin.,Hang, Wei.,Zhang, Hongjiang.,...&Chen, Fangjian.(2020).Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,50,8. |
| MLA | Zhang, Chunhui,et al."Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 50(2020):8. |
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