Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons

doi:10.1007/s00343-020-0040-7

	Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons
	Li Mengna 1,2,5; Chen Hao1,2,3,4 ; Wang Minxiao 1,2,3,4; Zhong Zhaoshan1,2,5 ; Zhou Li 1,2,3,4; Li Chaolun1,2,3,4,5
	2020-07-01
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
卷号	38 期号:4 页码:1292-1303
通讯作者	Li Chaolun([email protected])
摘要	Deep-sea mussels of the subfamily Bathymodiolinae are common and numerically dominant species widely distributed in cold seeps and hydrothermal vents. During long-time evolution, deep-sea mussels have evolved to be well adapted to the local environment of cold seeps and hydrothermal vents by various ways, especially by establishing endosymbiosis with chemotrophic bacteria. However, biological processes underlying the establishment and maintenance of symbiosis between host mussels and symbionts are largely unclear. In the present study,Gigantidas platifronsgenes possibly involved in the symbiosis with methane oxidation symbionts were identified and characterized by Lipopolysaccharide (LPS) pull-down andin situhybridization. Five immune related proteins including Toll-like receptor 2 (TLR2), integrin, vacuolar sorting protein (VSP), matrix metalloproteinase 1 (MMP1), and leucine-rich repeat (LRR-1) were identified by LPS pull-down assay. These five proteins were all conserved in either molecular sequences or functional domains and known to be key molecules in host immune recognition, phagocytosis, and lysosome-mediated digestion. Furthermore,in situhybridization of LRR-1, TLR2 and VSP genes was conducted to investigate their expression patterns in gill tissues ofG. platifrons. Consequently, LRR-1, TLR2, and VSP genes were found expressed exclusively in the bacteriocytes ofG. platifrons. Therefore, it was suggested that TLR2, integrin, VSP, MMP1, and LRR-1 might be crucial molecules in the symbiosis betweenG. platifronsand methane oxidation bacteria by participating in symbiosis-related immune processes.
关键词	Gigantidas platifrons endosymbiosis innate immunity pull-down assay immune recognition methane oxidation bacteria
DOI	10.1007/s00343-020-0040-7
收录类别	SCI
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000551529700026
出版者	SCIENCE PRESS
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168042
专题	海洋生态与环境科学重点实验室深海极端环境与生命过程研究中心
通讯作者	Li Chaolun
作者单位	1.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci CODR & KLMEES, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li Mengna,Chen Hao,Wang Minxiao,et al. Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(4):1292-1303.
APA	Li Mengna,Chen Hao,Wang Minxiao,Zhong Zhaoshan,Zhou Li,&Li Chaolun.(2020).Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(4),1292-1303.
MLA	Li Mengna,et al."Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.4(2020):1292-1303.

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