Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273 | |
| Wu, Z.1; Zheng, R.2; Zhang, J.2; Wu, S.1 | |
| 2020-07-16 | |
| 发表期刊 | JOURNAL OF APPLIED MICROBIOLOGY
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| ISSN | 1364-5072 |
| 页码 | 13 |
| 摘要 | Aims Relatively, few anti-biofilm polysaccharides againstPseudomonas aeruginosawere done to investigate the underlying molecular mechanism. Exopolysaccharide EPS273 can clearly reduce biofilm formation and infection ofP. aeruginosa. This study aims to investigate its anti-biofilm and anti-infection mechanism on transcriptional level. Methods and Results Herein, we used an RNA-Seq transcriptomic approach to investigate the underlying anti-biofilm and anti-infection mechanism of EPS273. The expression levels of a large number of genes were changed afterP. aeruginosaPAO1 was treated with EPS273. Especially, the genes related to biofilm formation, such as gene involved in production of extracellular matrix and virulence factor, genes involved in flagella and cell motility and genes involved in iron acquisition. Notably, the expression levels of genes involved in regulatory and signal transduction were markedly downregulated, such as two-component system PhoP-PhoQ and quorum sensing (QS) system LasI/LasR and RhlI/RhlR. Furthermore, when genesphoPandphoQwere disrupted, respectively, the reduction of biofilm formation and cell motility in mutant ophoPor ophoQwas also detected. Conclusion EPS273 may exert its anti-biofilm and anti-infection function by downregulating gene expression of two-component system PhoP-PhoQ and QS systems LasI/LasR and RhlI/RhlR ofP. aeruginosa, which further regulated expression of genes involved in biofilm formation. Significance and Impact of the Study Our data will expand understanding of anti-biofilm mechanisms of polysaccharides on transcriptomic level. |
| 关键词 | biofilm polysaccharide Pseudomonas aeruginosa transcriptomic virulence factor |
| DOI | 10.1111/jam.14764 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31600035] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000548698500001 |
| 出版者 | WILEY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167943 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wu, S. |
| 作者单位 | 1.Qingdao Univ, Coll Life Sci, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Z.,Zheng, R.,Zhang, J.,et al. Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273[J]. JOURNAL OF APPLIED MICROBIOLOGY,2020:13. |
| APA | Wu, Z.,Zheng, R.,Zhang, J.,&Wu, S..(2020).Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273.JOURNAL OF APPLIED MICROBIOLOGY,13. |
| MLA | Wu, Z.,et al."Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273".JOURNAL OF APPLIED MICROBIOLOGY (2020):13. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Transcriptional prof（2268KB） | 期刊论文 | 作者接受稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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