Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Functional characterization of retinoid X receptor with an emphasis on the mediation of organotin poisoning in the Pacific oyster (Crassostrea gigas) | |
Huang, Wen1,2 ; Wu, Qian2; Xu, Fei1,3,4; Li, Li1,3,4; Li, Juan1; Que, Huayong1,3,4; Zhang, Guofan1,3,4
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| 2020-08-30 | |
| 发表期刊 | GENE
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| ISSN | 0378-1119 |
| 卷号 | 753页码:7 |
| 摘要 | Marine mollusks suffer harmful effects due to environmental organotin compounds such as tributyltin (TBT) and triphenyltin (TPT). It is known that gastropod imposex caused by organotins is mediated by a key nuclear receptor, retinoid X receptor (RXR). The organotin-mediated toxic effects on oysters grown in seawater include a thicker shell, incomplete growth, disrupted development and a high rate of mortality. However, few studies have been conducted to determine the role of RXR in the toxic effects of organotins on bivalves. Here, we cloned an RXR homolog (CgRXR) from the Pacific oyster (Crassostrea gigas) and characterized its molecular function. Expression of the CgRXR RNA transcripts was assessed in whole developmental stages and tissues, with the highest expression detected in the blastula and mantle, respectively. The subcellular localization experiment confirmed that CgRXR protein was expressed in the nucleus exclusively as a nuclear receptor. Electrophoretic mobility shift assay indicated that CgRXR could bind to the DNA motifs DR0-DR5. The dual-luciferase reporter assay demonstrated that the transcriptional activity of CgRXR was activated by conserved ligands (9-cis retinoic acid and cis-4,7,10,13,16,19-docosahexanoic acid) and endocrine-disrupting chemicals (TBT and TPT). These results revealed the conserved gene function involved in protein localization, ligand binding and heterodimer formation with thyroid hormone receptor. However, the DNA binding properties of CgRXR differed from those of other invertebrate and vertebrate RXRs. CgRXR had the highest expression level in the blastula and mantle, and the disrupted development or shell malformation induced by organotins suggested a possible correlation of CgRXR with shell formation in bivalves. The results indicated the potential involvement of CgRXR in the toxic effects of organotins (TBT and TPT) through signaling pathway disruption. Functional characterization of CgRXR will help us better understand the endocrinology of bivalves. |
| 关键词 | Nuclear receptor Retinoid X receptor Organotin Crassostrea gigas Bivalve |
| DOI | 10.1016/j.gene.2020.144780 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Guangxi Natural Science Foundation[2017GXNSFAA198196]; Guangxi Natural Science Foundation[2016GXNSFBA380028]; National Natural Science Foundation of China[41866006]; National Natural Science Foundation of China[41776152]; National Natural Science Foundation of China[31372515]; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019Q11]; China Agriculture Research System[CARS-49] |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| WOS记录号 | WOS:000541914900003 |
| 出版者 | ELSEVIER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167811 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Xu, Fei; Que, Huayong |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Guangxi Univ, Sch Marine Sci, Nanning 530004, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Huang, Wen,Wu, Qian,Xu, Fei,et al. Functional characterization of retinoid X receptor with an emphasis on the mediation of organotin poisoning in the Pacific oyster (Crassostrea gigas)[J]. GENE,2020,753:7. |
| APA | Huang, Wen.,Wu, Qian.,Xu, Fei.,Li, Li.,Li, Juan.,...&Zhang, Guofan.(2020).Functional characterization of retinoid X receptor with an emphasis on the mediation of organotin poisoning in the Pacific oyster (Crassostrea gigas).GENE,753,7. |
| MLA | Huang, Wen,et al."Functional characterization of retinoid X receptor with an emphasis on the mediation of organotin poisoning in the Pacific oyster (Crassostrea gigas)".GENE 753(2020):7. |
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| Functional character（2958KB） | 期刊论文 | 作者接受稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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