Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea | |
Sun, Qing-Lei1,2 ; Zhang, Jian1,2,3; Wang, Min-Xiao3,4; Cao, Lei3; Du, Zeng-Feng3,5; Sun, Yuan-Yuan1,2; Liu, Shi-Qi6; Li, Chao-Lun3,4,7; Sun, Li1,2
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| 2020-05-01 | |
| 发表期刊 | MICROORGANISMS
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| 卷号 | 8期号:5页码:18 |
| 通讯作者 | Li, Chao-Lun([email protected]) ; Sun, Li([email protected]) |
| 摘要 | In the Formosa cold seep of the South China Sea (SCS), large amounts of methane and sulfide hydrogen are released from the subseafloor. In this study, we systematically investigated the microbial communities in the seawater-sediment interface of Formosa cold seep using high-throughput sequencing techniques including amplicon sequencing based on next-generation sequencing and Pacbio amplicon sequencing platforms, and metagenomics. We found that Sulfurovum dominated the microbial communities in the sediment-seawater interface, including the seawater close to the seepage, the surface sediments, and the gills of the dominant animal inhabitant (Shinkaia crosnieri). A nearly complete 16S rRNA gene sequence of the dominant operational taxonomic units (OTUs) was obtained from the Pacbio sequencing platforms and classified as OTU-L1, which belonged to Sulfurovum. This OTU was potentially novel as it shared relatively low similarity percentages (<97%) of the gene sequence with its close phylogenetic species. Further, a draft genome of Sulfurovum was assembled using the binning technique based on metagenomic data. Genome analysis suggested that Sulfurovum sp. in this region may fix carbon by the reductive tricarboxylic acid (rTCA) pathway, obtain energy by oxidizing reduced sulfur through sulfur oxidizing (Sox) pathway, and utilize nitrate as electron acceptors. These results demonstrated that Sulfurovum probably plays an important role in the carbon, sulfur, and nitrogen cycles of the Formosa cold seep of the SCS. This study improves our understanding of the diversity, distribution, and function of sulfur-oxidizing bacteria in deep-sea cold seep. |
| 关键词 | cold seep seawater-sediment interface sulfur-oxidizing bacteria Campylobacterota Sulfurovum |
| DOI | 10.3390/microorganisms8050687 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key Research and Development Project of China[2018YFC0310801]; Chinese Academy of Sciences[NMST-KEXUE2017K01]; National Natural Science Foundation of China[41806202]; Taishan Scholar Program of Shandong Province; Senior User Project of RV KEXUE[KEXUE2018G20]; Senior User Project of RV KEXUE[KEXUE2019GZ02] |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| WOS记录号 | WOS:000540222300068 |
| 出版者 | MDPI |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167775 |
| 专题 | 海洋生态与环境科学重点实验室 海洋地质与环境重点实验室 深海极端环境与生命过程研究中心 |
| 通讯作者 | Li, Chao-Lun; Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Deep Sea Res Ctr, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 6.Univ Amsterdam, Fac Sci, NL-1098 XH Amsterdam, Netherlands 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋研究所; 海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Sun, Qing-Lei,Zhang, Jian,Wang, Min-Xiao,et al. High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea[J]. MICROORGANISMS,2020,8(5):18. |
| APA | Sun, Qing-Lei.,Zhang, Jian.,Wang, Min-Xiao.,Cao, Lei.,Du, Zeng-Feng.,...&Sun, Li.(2020).High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea.MICROORGANISMS,8(5),18. |
| MLA | Sun, Qing-Lei,et al."High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea".MICROORGANISMS 8.5(2020):18. |
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