Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells | |
Li, Xiaosong1,2,3; Wu, Ning1,2; Chen, Yongqiang4 ; Tan, Jiaojiao1,2,3; Wang, Jing1,2; Geng, Lihua1,2; Qin, Yimin5; Zhang, Quanbin1,2,3
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| 2020-05-15 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| 卷号 | 151页码:545-553 |
| 通讯作者 | Zhang, Quanbin([email protected]) |
| 摘要 | In order to investigate the anti-fibrotic effect of different molecular weight (Mw) fucoidans on TGF-beta 1-induced mouse renal tubular epithelial cell (MTEC) mode. Oxidative degradation method was used to obtain fucoidans with different molecular weights and the reaction time, reaction temperature and the concentration of oxidants were investigated. Cell viability was detected by CCK-8, and EMT markers expression was detected by Westernbolt and Cell immunofluorescence assay. As a result, after chemical analysis of three independent batches of prepared samples, one batch of fucoidan sample (LHX 1-9) which chemical contents are similar but Mw ranging from 3.3 KDa to 49.3 KDa were selected to do further research. We found LHX1 (Mw - 3.3 KDa) and LHX 3-9 (Mw = 6.6 KDa, 8.3 KDa, 11.3 KDa, 14.9 KDa, 25.2 KDa, 35.4 KDa, 49.3 KDa) could resist the TGF-beta 1-induced depithelial-mesenchymal transition (EMT) by decreased expression of Fn and CTGF and maintained epithelial cell morphology in MTEC. However, the relationship between the Mw of fucoidans and their anti-EMT effect is not simply linear. Among the samples, LHX 1, 5 and 8 showed significant anti-EMT effects than others by deregulated Fn and CTGF expression on MTEC cells. (C) 2020 Elsevier B.V. All rights reserved. |
| 关键词 | Degradation Fucoidan Epithelial-mesenchymal transition Kidney fibrosis |
| DOI | 10.1016/j.ijbiomac.2020.02.068 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key Research and Development Program of China[2018YFD0901104]; Major Scientific & Engineering Projects of Innovation in Shandong Province[2019JZZY010818]; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2016190]; K. C. Wong Education Foundation, CAS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS记录号 | WOS:000528267400056 |
| 出版者 | ELSEVIER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167329 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Quanbin |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Eighth Peoples Hosp, Anorectal Surg, Qingdao 266100, Peoples R China 5.State Key Lab Bioact Seaweed Subst, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Xiaosong,Wu, Ning,Chen, Yongqiang,et al. Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,151:545-553. |
| APA | Li, Xiaosong.,Wu, Ning.,Chen, Yongqiang.,Tan, Jiaojiao.,Wang, Jing.,...&Zhang, Quanbin.(2020).Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,151,545-553. |
| MLA | Li, Xiaosong,et al."Degradation of different molecular weight fucoidans and their inhibition of TGF-beta 1 induced epithelial-mesenchymal transition in mouse renal tubular epithelial cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 151(2020):545-553. |
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