Comparison of effect of dietary supplementation with Haematococcus pluvialis powder and synthetic astaxanthin on carotenoid composition, concentration, esterification degree and astaxanthin isomers in ovaries, hepatopancreas, carapace, epithelium of adult female Chinese mitten crab (Eriocheir sinensis)

doi:10.1016/j.aquaculture.2020.735146

IOCAS-IR > 实验海洋生物学重点实验室

	Comparison of effect of dietary supplementation with Haematococcus pluvialis powder and synthetic astaxanthin on carotenoid composition, concentration, esterification degree and astaxanthin isomers in ovaries, hepatopancreas, carapace, epithelium of adult female Chinese mitten crab (Eriocheir sinensis)
	Su, Fang1,2,3,4 ; Yu, Wenjie1,2,3 ; Liu, Jianguo 1,2
	2020-06-30
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	523 页码:7
通讯作者	Liu, Jianguo([email protected])
摘要	Astaxanthin is widely used in aquaculture, being added to feeds to give desirable coloration to the farmed animals. Little is known about the different effects of natural and synthetic astaxanthin on Chinese mitten crab (Eriocheir sinensis). This study was conducted to compare the effects of dietary supplementation with Haematococcus pluvialis powder and synthetic astaxanthin on carotenoid composition, concentration, degree of esterification and astaxanthin isomers of E. sinensis. Six experimental diets were formulated including a basal diet, three diets containing H. pluvialis powder (0.2%, 0.4%, 0.6%) and two diets containing synthetic astaxanthin (0.13%, 0.16%), with each diet being fed to female crabs (average body weight of 85 +/- 5 g) in triplicate tanks with 12 crabs in each. After feeding for 30 and 60 days, the ovaries, hepatopancreas, carapace and epithelium of 3 crabs from each tank were taken for carotenoid analysis. Dietary supplementation with H. pluvialis powder and synthetic astaxanthin increased the astaxanthin concentration of E. sinensis in a dose-dependent manner. After 60 days, the astaxanthin concentration in body parts of crabs from high to low were ovaries (45.19-343.37 mg/kg), epithelium (14.98-37.74 mg/kg), carapace (12.86-21.71 mg/kg) and hepatopancreas (0.49-8.75 mg/kg). Astaxanthin in the epithelium was found in esterified form but in other body parts the astaxanthin was not esterified. All-trans astaxanthin was predominant in the ovaries, epithelium and carapace, and the proportion varied from 59.74% to 96.94%. (3S,3'S)-astaxanthin was mostly deposited in the ovaries of crabs fed with diets containing H. pluvialis powder and the proportion (85.18%-96.27%) increased significantly with increasing astaxanthin concentration of diets. Differences of astaxanthin isomers between diets and body parts indicated that isomers were deposited selectively and were likely to be accompanied by isomerization. To achieve the same effect on body astaxanthin concentration, synthetic astaxanthin required a higher level of addition than H. pluvialis powder. These results suggest that H. pluvialis powder is more effective in astaxanthin accumulation of E. sinensis, and also improves carotenoid and astaxanthin isomer composition of E. sinensis in comparison with synthetic astaxanthin.
关键词	Feed additive Crustacean culture Crab diets Carotenoids Astaxanthin isomer
DOI	10.1016/j.aquaculture.2020.735146
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[U1706209]; National Natural Science Foundation of China[31572639]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000526415400007
出版者	ELSEVIER
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167194
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Fujian Normal Univ, Fuqing Branch, Key Lab Coastal Basin Environm, Fuzhou, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Su, Fang,Yu, Wenjie,Liu, Jianguo. Comparison of effect of dietary supplementation with Haematococcus pluvialis powder and synthetic astaxanthin on carotenoid composition, concentration, esterification degree and astaxanthin isomers in ovaries, hepatopancreas, carapace, epithelium of adult female Chinese mitten crab (Eriocheir sinensis)[J]. AQUACULTURE,2020,523:7.
APA	Su, Fang,Yu, Wenjie,&Liu, Jianguo.(2020).Comparison of effect of dietary supplementation with Haematococcus pluvialis powder and synthetic astaxanthin on carotenoid composition, concentration, esterification degree and astaxanthin isomers in ovaries, hepatopancreas, carapace, epithelium of adult female Chinese mitten crab (Eriocheir sinensis).AQUACULTURE,523,7.
MLA	Su, Fang,et al."Comparison of effect of dietary supplementation with Haematococcus pluvialis powder and synthetic astaxanthin on carotenoid composition, concentration, esterification degree and astaxanthin isomers in ovaries, hepatopancreas, carapace, epithelium of adult female Chinese mitten crab (Eriocheir sinensis)".AQUACULTURE 523(2020):7.

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