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| Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells | |
Wang, Jing1,2,3,4 ; Zhang, Quanbin1,2; Li, Shuang1,2,3; Chen, Zhihang1,2,3; Tan, Jiaojiao1,2,3; Yao, Jianting1,2; Duan, Delin1,2,4
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| 2020-05-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| 卷号 | 150页码:304-314 |
| 通讯作者 | Duan, Delin([email protected]) |
| 摘要 | Diabetic nephropathy (DN) is the most common cause of end-stage renal disease (ESRD). Currently, approximately 20-40% of individuals with diabetes are diagnosed with DN. Mesangial cells (MCs) are critical for maintaining and regulating glomerular filtration, and the abnormal proliferation of MCs causes the accumulation of mesangial extracellular matrix (ECM), further promoting glomerular dysfunction and renal diseases. Low molecular weight fucoidan (LMWF) extracted from Saccharina japonica could alleviate DN, but the mechanism was not analysed. Based on the ability of LMWF to ameliorate the human renal mesangial cell (HRMC) injury caused by advanced glycation end products (AGEs), we identified fibronectin (FN) as the most obviously impacted protein in the ECM-receptor interaction by proteomic analysis. The co-localization of LMWF and FN indicated direct interaction between them, and surface plasmon resonance (SPR) analysis confirmed the specific binding with a K-D of 453.7 mu mol L-1. Positively charged protamine sulfate (PS) promoted the combination of LMWF and HRMCs and further enhanced the effect of LMWF on HRMC injury. Our results indicated that LMWF alleviates the HRMC injury caused by AGEs via binding FN and inhibiting the ECM-receptor interaction pathway. These results provide a foundation for the in-depth analysis of the mechanism of polysaccharide functions. (c) 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| 关键词 | Fucoidan ECM-receptor interaction Fibronectin |
| DOI | 10.1016/j.ijbiomac.2020.02.087 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Marine Science and Technology Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1]; Bilateral Joint Research Project between China and Japan[2017YFE0130900] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS记录号 | WOS:000525869500031 |
| 出版者 | ELSEVIER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167164 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Duan, Delin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Zhang, Quanbin,Li, Shuang,et al. Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,150:304-314. |
| APA | Wang, Jing.,Zhang, Quanbin.,Li, Shuang.,Chen, Zhihang.,Tan, Jiaojiao.,...&Duan, Delin.(2020).Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,150,304-314. |
| MLA | Wang, Jing,et al."Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 150(2020):304-314. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Low molecular weight（2307KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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